??? (Mosquitoes) ?.????? 19 ???? 412 ????

1
????????????????????????????????????????????
???????? ????? (???.??????????) ????????????????
?????????? ???. 7 ??.
?????
???
??
1
2
??? (Mosquitoes) ?.????? 19 ???? 412 ????
???? 2 ?????????? ??? - ???????????????????
-
??????????????????????
3

????????????????????????????????
???????????????????????????? ?????????????????????
?
?????? (Ae. aegypti, Ae. albopictus)
???????????????
???????????????? ????????? ????????????
???
??
???????????????????
??????????????? ??????????????????????????
???????????? DEN-1 ???? DEN-2 ???? DEN-3 ????
DEN-4 ???????????????????
????
???????????????????????????? 1
4
???????????? Dengue virus
?????????????? 1- 2 ?????
??????????????? 8 -12 ???
??????????????????
????? ???????????
?????????????? 5 - 8 ??? (3-15 ???)
????? ?????????????? ??????????????
???????????????????????????? ?.?? ???? 8
5
?????????????????????????????????????????????????
???????????????????????
???????/?????????????? ?????? ??????? ??????????? ?????? ??????? ??????????? ?????? ??????? ??????????? tourniquet test tourniquet test tourniquet test
???????/?????????????? ?????? 1 ?????? 2 ?????? 3 ?????? 1 ?????? 2 ?????? 3
sensitivity 73.3 90.5 85.5 53.3 90.6 98.7
specificity 93.3 89.2 87.9 75.8 77.8 74.2
?????????????????????? ????????? (false negative)
????????? ?? ??????????????????? ?? ???????????
?? ?????????? ?? ??????????????????????????
(???????????????????????????????????????)
????? ??????????????????????????????????????????
??? ???????????????? ???? 34
?????????????????? ???????????????????????????????
???????? ?????? ??????? ??????????????????
6
(No Transcript)
7
???????????????????????????????????????????

• ?????????????????????????????????????????
• - ???????? ??????? (???????, ??????????)
• - ?????????????????????????????????
• - ??????????/??????/?????????
  ???????????????????????
• - ????????????????????????????????????????
  ????
• 2. ??????????????????????????????????????????????
• 2.1 ??????????????????????????????????????????????
  ???????????????????????????? (IVM- Integrate
  vector management)
• 2.2.????????????? ????????????????????????????????
  ??? /?????????????????????????????????????????
• - ???????????????????????????????????????????
  ????????????????????????????/??????? ????? ????
  ??????????????????????????????????????????????????
  ????????
• - ???????????????????????????????????????????
  ???????????????????????????? ?????????????????????
  ???????????????????
• 3. ???????????????????????????????????

8
?????????????????????? Aedes aegypti ??? Ae.
albopictus ???????????????????
??????????????????????????????? ??????????
????? ???. (??????????) ?????????????????????????
?? ???. 7 ??
???????????????????????????????????????????????
Aedes aegypti ??? Ae.albopictus
??????????????????????????????????????????????????
?????????????????????????????????????????????????
????????????? ????????????????????????????????????
??????? 1 ??? 24 ??. ??????? Bioassay test
??????????????????????????????????????????

9
Bioassay test
Indoor Ae.aegypti -
Hang Heigh 1-1.5 m
Outdoor Ae.albopictus - Hang Heigh 1-1.5
m, lt25 m from street,10-15m from house
10
1.?????????? ULV (???????????)

• ???????? 1 ???????????????????????? Bioassay test
  ?????????? ULV

???????? Test X ??? ???????? () ???????? () ???????? () ???????? () ???????? () ???????? ()
???????? Test X ??? Ae.aegypti Ae.aegypti Ae.aegypti Ae.albopictus Ae.albopictus Ae.albopictus
???????? Test X ??? Knocked down ???? 24 ??. ??? Knocked down ???? 24 ??. ???
?.??????? 6X20 30 (25) 32 (26.6) 62 (51.6) 28 (23.3) 16 (13.3) 44 (36.6)
?.?????? 6X20 16 (13.3) 10 (8.3) 26 (21.6) 12 (10) 20 (16.6) 32 (26.6)
?.????? 6X20 4 (3.3) 14 (11.6) 18 (15) 22 (18.36) 20 (16.6) 42 (35)
??? 18X20 50 (13.8) 56 (15.5) 106 (29.4) 62 (17.2) 56 (15.5) 118 (32.7)
(t -0.329, d.f. 2 , p-value 0.774)
???????? ???????? 8 ??./??., ???????????
250-300 ??./????
11
???????? 2 ??????????????????? Bioassay test
?????????? Fogging
2.??????????Fogging (???????????)
???????? (??????) Test X ??? ???????? () ???????? () ???????? () ???????? () ???????? () ???????? ()
???????? (??????) Test X ??? Ae.aegypti Ae.aegypti Ae.aegypti Ae.albopictus Ae.albopictus Ae.albopictus
???????? (??????) Test X ??? Knocked down ???? 24 ??. ??? Knocked down ???? 24 ??. ???
?.???????? 6X20 42 (35) 38 (31.6) 80 (66.6) 24 (20) 8 (6.6) 30 (25)
?.???????? 6X20 22 (18.3) 56 (46.6) 78 (65) 36 (30) 12 (10) 48 (40)
?.???? 6X20 26 (21.6) 52 (43.3) 78 (65) 16 (13.3) 38 (31.6) 54 (45)
??? 18X20 90 (25) 146 (40.5) 236 (65.5) 76 (21.1) 58 (16.1) 132 (36.6)
???????? ??????????? 100-200 ??./???? (30
??????/????) ???????? 1 ???2
????????????????????????????????????
(t 4.422, d.f. 2, p-value 0.048)
12
3. ????????????????????????????

• ???????? 3 ?????????????????????????????? ULV

???????? (??????) ??????????????/??. ??????????????/??. ??????????????/??.
???????? (??????) ??????? ??????? ???? ()
?.??????? (67) 44 17 61.4
?.?????? (129) 35 15 57.1
?.????? (122) 46 10 78.3
????????? 41.7 14 66.4
13

• ???????? 4 ??????????????????????????????Fogging

???????? ??????????????/??. ??????????????/??. ??????????????/??.
???????? ??????? ??????? ???? ()
?.???????? (80) 47 13 72.3
?.???????? (120) 18 6 66.7
?.???? (80) 18 3 83.3
??? 27.7 7.3 73.5
???????????????????????????????????????????????Fog
ging??? ULV ?????????????????????????????????????
?? (t -4.749, d.f. 2 , p-value 0.042)
14
(No Transcript)
15
(No Transcript)
16
Table 1 Mortality rate of Ae. aegypti larvae
exposed to instant sachet
temephos 1SG collected from
LAO-Yasothorn Province
Exposure Time (hrs.) Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae ()
Exposure Time (hrs.) Yasothorn strain Yasothorn strain Bora Bora strain Bora Bora strain
Exposure Time (hrs.) Exp-1 Exp-2 Exp-1 Exp-2
24 90 2 100 5
48 100 12 100 22
72 100 24 100 36
Average () 99.67 12.67 100 21
t 21.00, df 2, p 0.002 t 21.00, df 2, p 0.002 t 8.81, df 2, p 0.013 t 8.81, df 2, p 0.013
Remark Exp-1 shaky instant-sachet Exp-2 dunk
instant-sachet
17
Table 2 Mortality rate of Ae. aegypti larvae
to instant sachet temephos 1 SG
provider by DDC, Ministry of
Public Health
Exposure Time (hrs.) Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae () Mortality rate of Ae. aegypti larvae ()
Exposure Time (hrs.) Yasothorn strain Yasothorn strain Bora Bora strain Bora Bora strain
Exposure Time (hrs.) Exp-1 Exp-2 Exp-1 Exp-2
24 92 3 100 5
48 100 11 100 24
72 100 25 100 35
Average () 97.33 13 100 21
t 18.07, df 2, p 0.003 t 18.07, df 2, p 0.003 t 8.98, df 2, p 0.012 t 8.98, df 2, p 0.012
Remark Exp-1 shaky instant-sachet Exp-2 dunk
instant-sachet
18
??????????????????????????????????????????????
???????????? 3
???????????? 2
???????????? 1
Onset 10 ?? 56
Onset 8 ??? 56
Onset 7 ?? 56
gt 28 ???
gt 28 ???
????????????????????????? 3 ????? Dx DF 12 ?? 56
????????????????????????? 1 ????? Dx DHF 8??56
????????????????????????? 2 ????? Dx DHF 9???56
?????????????2 ???????????? 3 ????? Dx DF 14 ??
56
Onset 12 ?? 56
?????????????????????????? gt28 ???
?????????????????????????????????????? ???????????
??????????????????????????
19
???????????????????????????????????????????????
???????????? 1 ?? Pt 5 ???
???????????? 2 ?? Pt 2 ???
??????????????????? gt 28 ???
(?) ?????????????????????????????? (11, 13, 24
??? 56)
(?) ??????? ?????????? ???????????? 1
????????????? ??????????? (?????????2??56)
(?) ????????????????????? ????????? 2
??????????????? 9???56 ?????? 2 ????????? 15 ???
56
(?) ?????????????????????????????????????
??? (????????? 7???56)
?????????????????????????? gt28 ???
?????????????????? ???????????????????????????????
? 1 ????????????????????
20
??????????????????????????????????????????????
???????????? 1 ?? Pt 5 ??? ????pt gen 2
???????????? 2 ?? Pt 2 ???
??????????????????? gt 28 ???
(?) ?????????????????????????????? (11, 13, 24
??? 56)
(?) ????????????????????? ????????? 2
??????????????? 9 ??? 56 ?????? 2 ????????? 15
??? 56
(?) ??????? ?????????? ???????????? 1
????????????? ??????????? (?????????6??56)
(?) ?????????????????????????????????????
??? (????????? 7???56)
?????????????????????????? gt28 ???
?????????????????????????????????????? ???????????
? 1 ????????????????????
21
??????????? 34010145 ???????????? ??? ??? Gen ??????????????? Pt ?????? ???????????? 28 ??? ??????????
Event 1 17/5/2013 1 14/6/56 14/6/56
Event 2 27/6/2013 2 24/7/56 24/7/56
Event 3 4/8/2013 1 1/9/56 1/9/56
12/8/2013 1 8/9/56
15/8/2013 1 11/9/56
16/8/2013 1 12/9/56
??????????? 34010145 ?? 3 event 7 ?? pt ??????? Gen 1
22
??????????? ???????????? ??? ??? Gen ??????????????? Pt ?????? ???????????? 28 ??? ??????????
34010818 16/6/2013 1 14/7/56 14/7/56
Event 1 2/7/2013 1 29/7/56
8/7/2013 1 3/8/56
17/7/2013 1 12/8/56
?? Pt ?????? Gen 2
Event 2 14/8/2013 1 10/9/56 10/9/56
17/8/2013 1 12/9/56
?? pt ??????? Gen 1
??????????? 34010818 ?? 2 event 6 ???? Gen 2 ??event 1
23
34010818 16/6/2013 1
  2/7/2013 1
  6/7/2013 1
  8/7/2013 1
  17/7/2013 1
  23/7/2013 1
  24/7/2013 1
  30/7/2013 1
  15/8/2013 1
  19/8/2013 1
  22/8/2013 1
  30/8/2013 1
  2/9/2013 1
  11/9/2013 1
34010818 ?????   14
24
????????????????????????????
????????? 1
???????????????????????? 9 ??? 56
????? 24 ??? 56 ????????? Pt ???????
14 ???????????????????????????????????????????????
???
(?) ???????????????????????????????????????? (onse
t 7 ??? 56)
25
??????????????????????????????????????????

• ?. ????????????????????????????/
  ??????????????????
• ?. ??????????????????????????????
  ??????????????????????????????????????? 100 ????
  (???????) ????????????????????????????????????????
  ?? ???????????????? ????? HI 0
• ?. ????????????????????????????
  ???????????????????????????????????????? 100 ????
• ?. ????????????????????? 2 ?????????????????????
  ? 7 ???
• ?. ????????????????/???????????????? 28
  ??????????????????????????????????????????????????
  ???

26
?????????????????????????????

• ??????????????????????? ????????????? WHO
  ??????
• ??????????????????? 100 ???? ???????????????????
  ???? ????
• ???????
• ???????????????? 100-199 ???? ????? 45
  ???????????
• ???????????????? 200-299 ???? ????? 51
  ???????????
• ???????????????? 300-399 ???? ????? 54
  ???????????
• ???????????????? 400 ???? ????? 55
  ???????????

????? World Health Organization. Prevention
and Control of Dengue and Dengue Haemorrhagic
Fever. Regional Pulbication, SEARO. New Delhi,
1999.
27
?????????????????32
28
???????????????? 100 ?.
29
(No Transcript)
30
(No Transcript)
31
?????????