???A

1
????????????????????????????????

• ???A
• ???
• 4960N056

2
??

• ??????????????????????????????,???????????????
  ??????????????????????????????????????????????????
  ?(??????(poly-hydroxyalkanoate),
  PHA)??????????????(Eisenia fetida)????,???????????
  ???,??????????PBS ????????,?14 ???????????????

3

• ????????????2 ?????????????,???????(5
  g/L)??????,????????????????PHA????????????????????
  ?????????????????????(Aeromonas hydrophila NIU01
  ?YTl1 (Chen et al., 20082009)?????(5
  g/L)????????????PHA??????,????????????????????????
  ????????

4
?????????????

• 1.????????????,?Filter Paper Method(Dalby et
  al., 1996)?????????,????????8200 unit/L?????????
• 2.????
• 3.??????????????????????

5

• 4.??????????????5 mL?????,??1???,???????????????
  ,???????????,????????????,?30??125 rpm ???????12
  ??,??????????50 mg/L?,?????????????????????

6
????????????

• 1.????????????????????,????????????????
• 2.??????????-?????????????PBS ????????20-24???,??
  ?40 (v/v)?????????

7

• 3.?????????????-?????????????PBS ????????20-24
  ???,???5 mL????100 mL MR ????500 mL????,???1 mL
  TES-I?0.1 mL TES-II,30?,200 rpm,72
  ????????,??????4???????????,????(ADVANTEC,NO.5A,7
  µm)??????????,???????????(6000 rpm,10
  ??),???100???8 ????,???????????,????PHA???

8

• 4. PHA????PHA???????????(gas chromatography,SRI
  8610C,USA)?????

9
?????
10
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11
(No Transcript)
12
(No Transcript)
13
(No Transcript)
14
??

• ???????????(??-PBSMM)????? (Chen et al.,
  20082009 2010)?????????(LB)????,????????????????
  ??????????????????????????,????????,??????????????
  ?,?????????????,?????? (Chen et al., 2008 2009
  2010)????????(LB)?????????

15

• ?????????????,????????????PHA??????,???????-??
  ????????????PHA????????(2- RR198?2- RB5?3-
  RBu198?4- RR198),????Nile red??? (Spiekermannet
  al., 1999)?????????,? UV ??????????????????,??????
  ??,??????????(lipid)???,?????????PHA??????????????
  ?????????,????????????????????????????????????????
  

16
????

• 1. B. Y. Chen, W. M. Chen, F. L. Wu, P. K. Chen
  and C. Y. Yen, Revealing azo-dyedecolorization
  of indigenous Aeromonas hydrophila from fountain
  spring inNortheast Taiwan, J. Chin. Inst. of
  Chem. Engrs., 39, 495-501 (2008).
• 2. B. Y. Chen, K. W. Lin, Y. M. Wang and C. Y.
  Yen, Revealing Interactive Toxicityof Aromatic
  Amines to Azo-dye Decolorizer Aeromonas
  hydrophila. Journal ofHazardous Materials, 166,
  187-194 (2009).
• 3. P. A. Pekakis, N. P. Xekoukoulotakis,
  D.Mantzavinos., Treatment of textiledyehouse
  wastewater by TiO2 photocatalysis, Water Res.,
  40, 12761286 (2006)
• 4. Youn-Joo An, Assessing soil ecotoxicity of
  methyl tert-butyl ether using earthworm bioassay
  closed soil microcosm, Environmental Pollution,
  134,181-186 (2005).