BIO 225 Microbiology

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BIO 225 Microbiology

• Ken Malachowsky
• Florence-Darlington Technical College

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MICROSCOPE
Illuminates
Magnifies
Contrasts
Optics
Resolves
SPECIMEN
Distinguishes
Lens systems
Background
Ability to
Vary Light intensity
Condenser
OCULAR
OBJECTIVE
Provide sharp, crisp image
Lamp
Distinguish
Total magnification
Two close objects
Iris diaphragm
Multiply two powers
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Leewenhoeks Simple Microscope
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A Compound Brightfield Microscope
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Scanning Power
X
4x
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Low Power
X
X
4x
10x
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High Power
X
X
4x
10x
40x
4x
10x
40x
100x
Field of Vision
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(No Transcript)
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Depth Perception
Steve Berg winona state university, used
with permission
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Refraction
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The use of Immersion Oil
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Microscope Rules

• Never force anything on the microscope
• Inform the instructor if there is a problem with
  the microscope
• Only use your assigned microscope
• Please do the following when you are finished
• Click the 4x objective in place
• Lower the stage far from the objective
• Place the microscope in its assigned space

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Slides to View

• Letter E
• Colored Threads
• Cheek cells
• Arthropod Cyclops or Daphnia
• Algae Volvox, Spirogyra, or Stentor
• Prototists Amoeba, Paramecium or diatoms
• Mixed Bacteria
• Pond water wetmount

KM
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Cheek Cells
100x
400x
KM
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Slides to View

• Letter E
• Colored Threads
• Cheek cells
• Arthropod Cyclops or Daphnia
• Algae Volvox, Spirogyra, or Stentor
• Prototists Amoeba, Paramecium or diatoms
• Mixed Bacteria
• Pond water wetmount

KM
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Cyclops
100x
KM
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Daphnia
100x
KM
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Volvox
100x
400x
KM
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Spirogyra
400x
KM
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Amoeba
100x
400x
KM
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Paramecium
100x
400x
KM
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Representative algae, or plantlike protists The
diatom Campylodiscus hibernicus
These protists usually have cell walls surrounded
by a loosely attached test that contains silicon
or calcium carbonate. Most reproduce by binary
fission. Some lack flagella.
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Diatoms
400x
KM
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Mixed Bacteria
1000x
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Slides to View

• Letter E
• Colored Threads
• Cheek cells
• Arthropod Cyclops or Daphnia
• Algae Volvox, Spirogyra, or Stentor
• Prototists Amoeba, Paramecium or diatoms
• Mixed Bacteria
• Pond water wet mount