CYTOBAND-BASED GENE SET ENRICHMENT ANALYSIS DETECTED SPARCL1 AS A MOLECULAR MARKER OF COLORECTAL CANCER LIVER METASTASIS - PowerPoint PPT Presentation

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CYTOBAND-BASED GENE SET ENRICHMENT ANALYSIS DETECTED SPARCL1 AS A MOLECULAR MARKER OF COLORECTAL CANCER LIVER METASTASIS

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Title: CYTOBAND-BASED GENE SET ENRICHMENT ANALYSIS DETECTED SPARCL1 AS A MOLECULAR MARKER OF COLORECTAL CANCER LIVER METASTASIS


1
CYTOBAND-BASED GENE SET ENRICHMENT ANALYSIS
DETECTED SPARCL1 AS A MOLECULAR MARKER OF
COLORECTAL CANCER LIVER METASTASIS ????????????SP
ARCL1????????
???,???,???,??,???,?? ??????????????????,?????????
????????
2
????
  • ?????????????
  • --??? 35?
  • --??? 45?
  • ?????? -- ???????????
  • ??--????????????
  • ????,??????510??
  • ???????(??) ???3681????

3
????
  • ???????????
  • ???????????
  • ????????
  • ???????????????????????????

Brabletz T, Nature Rev Cancer 2005
4
?????
  • ???????,????Affymetrix
  • ??????????? ??????????
  • ??????? ????????????????

???? ???
??????? 13
???????? 12
5
  • GSEA Gene Set Enrichment Analysis,????????
  • ?????????,????????????????????????????????
  • ???????????Pathway???, ?????Cytoband???
  • ????????????????????????

Subramanian et. al, PNAS, 2005
6
????? ??????
  • ????????GSEA????
  • 4q22?????????????,????????????????????????
  • SPARCL1???4q22???????(????)

7
SPARCL1????
  • ????????4q22,?????35 kb,?11????? 10??????
  • mRNA???3 kb,??664????,???????? 71 kDa,?????????
  • ???????????,??????????,?????,????,?????? B
    ???????
  • ?????????????????????????????????????,????????
  • SPARCL1 mRNA???,SPARCL1???????
  • ?????, ??????, ??????????

8
SPARCL1?????????????
  • ??? ?????? ????
  • ??? ?????
  • ????,P0.007

???SPARCL1 mRNA????
9
SPARCL1?????????????
  • ??? ?????? ????
  • ??? ?????
  • ????,P0.004
  • SPARCL1???????mRNA??

SPARCL1?????????
10
  • SPARCL1 ???????????
  • ???? His ?????????pET-22b-SPARCL1
  • ????????????????pLXSN-SPARCL1
  • ????????,???????????????
  • SPARCL1 ?????????
  • pET-22b-SPARCL1 ?????????
    E.coli(BL21(DE3)/Polys)
  • ?????? IPTG ????????
  • Ni2?????????

11
SPARCL1????
  • ?????????SPARCL1
  • ????????????
  • ?? ???????

12
SPARCL1???????
  • ????
  • 175???????
  • P 0.025
  • SPARCL1???????

SPARCL1???????
13
???????????
  • ????????
  • P53?MAPK? E-cadherin?MSH2?ADCY-2?Shp2?SPARCL1
  • ????
  • ?????
  • P53SPARCL1??????????

14
  • SPARCL1??????????????
  • SPARCL1?????????????????????
  • SPARCL1????????????????,??????????P53?????????????
    ???
  • SPARCL1??????,????????
  • ????????????????????

15
  • ??
  • ??? ????,mRNA????
  • ???????,????
  • ???????,????,????,????
  • ??????,??????
  • ????????????,ELISA

??
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