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Absorption Spectra

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Science is facts; just as houses are made of stone, so is science made of facts; but a pile of stones is not a house, and a collection of facts is not necessarily ... – PowerPoint PPT presentation

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Title: Absorption Spectra


1
Science is facts just as houses are made of
stone, so is science made of facts but a pile of
stones is not a house, and a collection of facts
is not necessarily science. Jules Henri Poincaré
(1854-1912) French mathematician.
Absorption Spectra of Chlorophyll
2
Electromagnetic Spectrum
3
Measuring Light
  • Irradiance measure of the amount of energy
    falling on a flat surface (amount per unity area)
  • Intensity the amount of light leaving a light
    source
  • Photon particle of light
  • Quanta energy in a photon
  • PPFD Photosynthetic Photon Flux Density (amount
    per unit area)
  • PAR photosynthetically active radiation
  • (350nm-700nm)

4
Measuring light
  • Photometric
  • Based on our eyes ability to detect
    electromagnetic radiation
  • Units are foot-candles (fc) or lumens m-2 (lux)

5
Measuring light
  • Radiometric
  • Based on actual spectral distribution
  • Units are
  • Energy W m-2
  • Number of Photons umole-m-2-s-1

Why do physiologists like photons?
6
Photosynthesis 1. Conversion of light energy
to chemical energy. 2. Short term storage of
chemical energy. 3. Long term storage of
chemical energy.
7
Basic principle of Photo-Chemistry
One photon-------One Excitation
8
Absorption Spectra of Chlorophyll
9
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10
Questions to answer using figure 1.
  • What is the effect of the atmosphere on direct
    sunlight?
  • Look at the Direct Sunlight at Sea Level line.
    What causes all the dips in the infra-red?
  • What colors (wavelengths) of light are
    selectively adsorbed by clouds?
  • What color is sky light?
  • Can you tell from this graph why leaves are
    green?
  • What is the significance of the lack of
    absorbance of infra-red radiation by vegetation?

11
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12
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