25. What is the proper order for the PCR cycle?

1

• 25. What is the proper order for the PCR cycle?
• A) Annealing, denaturation, extension
• B) Denaturation, Annealing, Extension
• C) Extension, Annealing, Denaturation
• D) Extension, Denaturation, Annealing
• E) None of the above

2

• 26. What chemicals are required to run PCR?
• Primers
• Nucleotides
• DNA polymerase
• Amino acids
• Ethanol

1. 1, 2 and 3 only
2. 1, 3 and 5 only
3. All
4. 3 and 4 only
5. 2, 3 and 4 only

3

• 27. When placing DNA into the gel, always place
  the side with the wells next to the __________
  electrode.
• Negative
• Positive

4

• 28. Restriction enzymes are used to
• Make the DNA heavy for the gel
• Amplify the DNA
• Break up the proteins bound to the DNA
• Filter out impurities
• Cut the DNA in specific places

5

• 29. In the fish experiment, what is being used as
  the positive control?
• Water
• Ethanol
• Histone proteins
• Salmon DNA
• Primer mix

6

• 30. In the fish experiment, what is being used as
  the negative control?
• Water
• Ethanol
• Histone proteins
• Salmon DNA
• Primer mix

7

• 31. What piece of equipment is used to load the
  DNA into the gel?
• Beaker
• Graduated cylinder
• Micropipette
• Fingers
• None of the above

8

• 32. What did we put on top of the solution just
  prior to placing the PCR micro-tubes into the PCR
  machine, to prevent evaporation from occurring?
• DNA
• Water
• Oil
• Ethanol
• None of the above

9

• 33. What size fragments move the fastest through
  the gel?
• Smaller
• Larger
• They all move the same speed

10

• 34. During the DNA extraction portion of the lab,
  what chemical was used to wash the DNA 3x?
• Water
• Elution Buffer
• Ethanol
• PCR mix
• None of the above