Aspergillus

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Aspergillus Classic Diagnostic

Dr. Patricio Godoy Martínez Universidad Austral
de Chile pgodoymartinez_at_gmail.com
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Aspergillus

• Ubiquitous, commonly ocurring in soil, water, and
  decaying vegatation.
• Reservoirs in hospitals unfiltered air,
  ventilation system, carpeting, food, water
  distribution system, etc.
• Cause invasive and allergic disease in humans and
  other animals.
• Cause plant and food spoilage and produce
  mycotoxins.

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Classification of Aspergillus. 260 especies, 38
have caused disease
Balajee Marr,2006. Future Microbiology,
1435-445.
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Sexual stages associated with the genus
Aspergillus
Geiser 2009. Medical Mycology, 47S21-S26
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Aspergillus
Mycology
Diagnostics
Indirect
Direct
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Aspergillus
Direct Methods
Colony
Microscopic examination
Detection of Ag and A.N
Coloration Histopathology
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Direct microscopic examination

• hyphaes hyalines septate.

Vitale R
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Histopathology HE
Vitale R
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Histopathology Grocott
Vitale R
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• Identification

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Macro-microscopic observation of colonies
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Morphological character
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Morphological character
COLUMNAR
RADIATE
Josep Guarro
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Aspergillus fumigatus
Colonies black blue-green, consisting of a dense
felt of conidiophores.
www. pgodoy. com
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Aspergillus fumigatus
Conidial heads columnar. Conidiogenous cells
uniseriate. Conidiophore stipes smooth-walled
often green in the upper part. Conidia verrucose.
www.pgodoy.com www.aspergillus.man.ac.uk
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Neosartorya

• Anamorphs closely related to A. fumigatus.
• Teleomorphs only differentiated by subtle
  differences in ascospores ornamentation.
• Thermophilic.

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Neosartorya

• N. fischeri
• N. pseudofischeri
• N. hiratsukae

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Neosartorya

• Osteomyelitis
• Keratitis
• Endocarditis
• Peritonitis
• Cerebral infection
• Pulmonary infection
• Disseminated infection

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Neosartorya pseudofischeri
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Neosartorya hiratsukae
Josep Guarro
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N. hiratsukae
N. pseudofischeri
Josep Guarro
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Josep Guarro
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Neosartorya

• Underdiagnosed (regarded as contaminants in the
  lab)
• Difficult to identify at species level.
• Antifungal susceptibility similar to A. fumigatus
  ?
• Little experience in the treatment. Amphotericin
  B and itraconazole

Josep Guarro
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Aspergillus niger
Colonies black, consisting of a dense felt of
conidiophores.
www. pgodoy. com
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Aspergillus niger
Conidial heads radiate. Vesicles subspherical,
conidiogenous cells biseriate. Conidia brown,
ornamented.
www.aspergillus.man.ac.uk
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Aspergillus flavus
Colonies yellowish-green, consisting of a dense
felt of conidiophores.
www.aspergillus.man.ac.uk www.pgodoy.com
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Aspergillus flavus
Conidial heads radiate. Conidiogenous cells uni
and biseriate. Conidiophore stipes rough-walled
hyaline. Conidia echinulate. Sclerotia may be
present.
www.aspergillus.man.ac.uk
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Aspergillus parasiticus
R. Salim R. Runco
Colonies yellowish-brown, consisting of a dense
felt of conidiophores.
conidiophores of varying lengths, wall hyaline,
thick, with ornamentation equinulada, spherical
vesicles, uni or biseriate (24-25 m), completely
covering the fiálides gallbladder. Conidia
globose or subglobosos warted (2.5-3 m) with thin
walls (20, 21).
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Aspergillus tamarii
Conidial heads compact and spherical or loosely
radiate. Conidiogenous cells uni and biseriate.
Conidiophore stipes rough-walled hyaline. Conidia
echinulate.
www.aspergillus.man.ac.uk
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Aspergillus orizae
Conidial heads radiate to loosely columnar.
Conidiogenous cells uni and biseriate.
Conidiophore stipes rough-walled hyaline. Conidia
smooth-walled to roughened.
www.aspergillus.man.ac.uk
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Aspergillus terreus
Colonies yellowish-brown to cinnamon-brown,
consisting of a dense felt of conidiophores.
www.aspergillus.man.ac.uk
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Aspergillus terreus
Conidial heads densely columnar. Conidiogenous
cells biseriate. Conidiophore stipes
smooth-walled hyaline. Conidia smooth walled,
striate hyaline.
www.aspergillus.man.ac.uk
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Aspergillus nidulans
Colonies growing rapidly, green, cream-buff or
honey-yellow, reverse dark purple.
Vitale R
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Aspergillus nidulans
Conidial heads short, columnar. Conidiogenous
cells biseriate. Conidiophore stipes brownish.
Conidia spherical, rugulose, subhyaline, green.
Vitale R
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Aspergillus clavatus
Colonies growing rapidly, bluish-green,
consisting of a dense felt of conidiophores.
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Aspergillus clavatus
Conidial heads radiate, later splitting into
several columns. Conidiogenous cells uniseriate.
Conidiophore stipes smooth. Vesicle clavate.
Conidia smooth-walled hyaline.
www.pgodoy.com
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INFOCUS 2010 - CHILE