b. The second round of global phrap assembly was done on April 1, 2002 on 77,022 Project ESTs. Of these, 70,074 were 5' ESTs and 6,948 were 3' ESTs. They were assembled into 11,758 contigs.

1
DBI-9975989
Contract Agreement DBI-9975989
b. The second round of global phrap assembly was
done on April 1, 2002 on 77,022 Project ESTs. Of
these, 70,074 were 5' ESTs and 6,948 were 3'
ESTs. They were assembled into 11,758 contigs. c.
ESTs selected from each contig and those of
unassembled ESTs are the resources for singleton
selection. Altogether, about 32,000 ESTs are in
this resource pool for further screening. d. ESTs
containing sequences found to match
retro-elements, E. coli, phage, mitochondrial,
and chloro-plast gene sequences are removed.
Sequence com-parison is done using the
cross_match program. e. Validation
processRedundant ESTs are further screened and
removed by comparing 3' sequence data with 3'
sequences of previously identified sing-letons.
The resulting singletons were rearrayed for probe
distribution. 2. Mapping results As of Aug. 27,
8,789 probes have been sent out to the 10 labs
and mapping data have been returned for 46 of
the distributed probes. At Albany, map-ping data
are processed to display the mapped probes by
chromosome bin position, defined by the deletion
line break points. The Project has assigned a
coordinator from among the investigators for each
homoeologous chromosome group who review and
validate the assignments of the probe locations.
Each probe may identify more than one loci and,
at this point, the validated probe locations
account for 7,985 individual loci mapped to
chromosome bins.
Bioinformatics personnel Data Curator Shiaoman
Chao, based at Albany, and Bioinformatics
Pro-grammer Hugh Edwards, based at Cornell Univ.,
are supported by collaboration with USDA ARS
bioinformatics specialists in Albany (G. Lazo)
and at Cornell (D. Matthews).