B a c t i F l o w

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B a c t i F l o w
Compact Microbial Analyser
Real-time microbiology analysis in Food,
beverages cosmetics
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Based on Proven Technologies

• Fluorescent cell labelling (Fluorassure)
• Same reagent technologies as used on D-Count
  ChemScan RDI
• Validated on more than 500 strains
• Bench Top Microbial Analyser
• Compact Flow cytometer specifically designed for
  microbial detection
• Laser based detection
• Simple to operate
• Data acquisition, analysis and real time result
  display
• Automatic data interpretation
• Quantitative results in minutes

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FluorassureÒ Viability Markers
Viability substrate

• Criteria of Viability
• Enzyme activity
• Membrane integrity

Enzyme
Bacteria
Free fluorochrome

• Applicable for
• Total Viable Count
• Yeast Moulds
• Specifics (on going)

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Quantitative Microbial Counting

• Flow Cell
• Linear fluid stream
• Cells Separated into
• single entities
• Sensitive detectors

Labelled Sample
Detector 1
Laser
Detector 2
B a c t i F l o w
Sample
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Pure Culture Correlation
Combined plot of all micro-organisms analysed
Staphylococcus aureus Escherichia coli Bacillus
circulans Bacillus licheniformis Enterobacter
cloacae Candida albicans Penicilium
decumbeus Aspergillus niger
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R a p i d

• Time to result
• 10 minutes labelling prior to analysis
• 1 minute analysis per sample
• Simple sample preparation adapted to product
  matrices

No cell multiplication required
Labelling 10 min.
Counting 1 min.
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S e n s i t i v e

• Combining Speed with Sensitivity
• Quantitative counting cell by cell
• Sensitivity of detection less than 100 cells /
  gram.
• Linear response up to 100,000 micro-organisms
• Direct Counting in less than 15 minutes
• Protocol available for Presence / Absence within
  24 hours

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E a s y t o U s e

• Ready to use Reagents
• Simple preparation for sample products
• Validated protocols for a full range of
  applications
• Fully automated detection and counting
• No data interpretation required
• Full data traceability
• PC with Windows XP interface

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B a c t i F l o w A p p l i c a t i o n s
Dairy Products
Cosmetics
Fruit based Products
Water
Pure cultures