Wet Mount PT: 2007B Specimen 1

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Wet Mount PT 2007BSpecimen 1

• Evaluate micrographs 1-a, 1-b, and 1-c and
  together as if different fields from the same
  patient
• Identify cellular images labeled as
• 1
• 2
• 3
• Enter results on result sheet

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Micrograph 1-a High Power
3
1
2
2
3
1
3
Micrograph 1-b High Power
1
3
2
2
4
Micrograph 1-c High Power
2
1
1
3
5
Wet Mount PT 2007B Specimen 2

• Evaluate micrographs 2-a, 2-b, and 2-c together
  as if different fields from the same patient
• Identify cellular images labeled as
• 4
• 5
• 6
• Enter results on result sheet

6
Micrograph 2-a Low Power
5
5
4
4
7
Micrograph 2-b Low Power
4
4
6
5
4
8
Micrograph 2-c High Power
4
6
4
5
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Wet Mount PT 2007B Specimen 3

• Evaluate micrographs 3-a, 3-b, and 3-c together
  as if different fields from the same patient
• Identify cellular images labeled as
• 7
• 8
• 9
• Enter results on result sheet

10
8
7
9
Micrograph 3-a Low power
11
8
7
7
8
Micrograph 3-b Low Power
12
Micrograph 3-c High Power
9
9
9
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Wet Mount PT 2007B Educational Challenge

• Evaluate the following micrographs
• These will not be graded!

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Phase Contrast High Power
Identify this cell
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(No Transcript)
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1a Healthy Trichomonas 1b Dying
Trichomonas (rounded up)
For size reference 2Squamous Epi 3 Yeast
cell
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Trichomonas vaginalis
Flagella
Nucleus
Flagella
Nucleus
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Trichomonas

• Trichomonas are parasitic protozoa.
• They can be very motile
• When they begin to die (within 10 minutes of
  specimen collection), they become sedentary and
  begin to round up.
• Cells also fatten when grown in culture medium
  (as in the previous slides).
• Trichomonas should only be reported when motility
  is observed

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Trichomonas have a very complex structure. They
have four flagella facing forward and a fifth
facing backward which is attached to an
undulating membrane. Sometimes, an axiostyle
or structural shaft can be seen often it
cant The cells are oval in shape, 10-23 µm in
length
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Assessment of Cell Size

• Use nucleus of Squamous Epi Cell as size marker
• Nucleus 15 microns
• Yeast 5-7 microns
• RBC 6-8 microns
• WBC 15 microns
• Trichomonas 20 microns