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Riboswitch Functions

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an expression platform for gene regulation. A metabolite-binding aptamer domain and Riboswitch Functions U U U U U X 3 4 2 1 Direct (protein-free ... – PowerPoint PPT presentation

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Title: Riboswitch Functions


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Riboswitch Functions
  • Direct (protein-free) binding of metabolite to
    RNA
  • Metabolite-dependent regulation of genes

These two activities are accomplished by two
functionally separate domains on the RNA
X
3
4
2
1
U
U
U
U
U
Courtesy Ron Breaker Lab (Yale University)
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Riboswitch Mechanisms
They also affect RNA integrity, and perhaps
splicing and stability.
Ligand binding leads to mRNA cleavage by a new
natural ribozyme.
X
Ligand binding could control splicing in
eukayotes.
Possibly, ligand binding to the 3 untranslated
region could affect mRNA stability.
Courtesy Ron Breaker Lab (Yale University)
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Riboswitch Ligands
There are 8 confirmed riboswitches with unique
metabolite ligands.
Many more conserved RNA motifs are currently
under investigation.
Courtesy Ron Breaker Lab (Yale University)
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Courtesy Ron Breaker Lab (Yale University)
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Manufacturing of Affy GeneChip
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Affymetrix E. coli GeneChip Image
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