Invitro Testing of Anti-Biofilm Burn and Wound Care Formulations

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Invitro Testing of Anti-Biofilm Burn and Wound
Care Formulations

• Scott Tufts, Vice President
• Cardinal Health, Infection Prevention
  

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Acknowledgements

• Dr. Paul S. Attar, President, Bridge PTS
  (Preclincal Testing Services), San Antonio, TX
• Mr. Kan Lam, Vice President, Bridge PTS
  (Preclincal Testing Services), San Antonio, TX
• Miss Yanira Hernandez, Scientist, Enturia, Inc.

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The Way We Used to Think About Bacteria
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The Real World of Chemotactic Communication
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Bean Sprouts with E. coli O157H7
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E coli on Spinach
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Biofilms on Fresh Vegetables

• According to statistics from the Centers for
  Disease Control and Prevention (CDC) in Atlanta,
  only 0.6 percent of disease outbreaks from food
  in the 1970s could be traced to fresh produce.
  However, since 1998, the percentage has jumped
  up to 14.

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Now We Know About the EPS
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The Biofilm Process
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Biofilms and Human Disease

• It is estimated that over 80 of all microbial
  infections of the human body are associated with
  biofilm structure for example
• Acne
• Tuberculosis
• Periodontal Disease
• Pneumonia
• Osteomyelitis
• Ear, Nose, and Throat Infections
• All Catheter and Percutaneous Device Infections
• All Wound and Burn Infections

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Problems Presented by Biofilms

• It is difficult to deliver antimicrobial agents
  through the exopolysaccharide matrix at effective
  concentrations. Concentrations required to
  penetrate the biofilm are thousands of times
  greater than necessary to treat planktonic
  bacteria. Often higher than the toxicity
  threshold of the antibiotic!
• The cells at the base of the biofilm are
  operating at a very low metabolic rate, they are
  hibernating, waiting for a nutrient source to
  revive them. This low metabolic rate of the
  sessile organisms, drastically reduces the
  effectiveness of antibiotics and promotes
  antibiotic resistance.

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Infected Burns and Wounds
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Testing Objectives

• Testing Objective / Challenge
• To utilize laboratory methods to screen and
  evaluate trial formulations for their potential
  to eliminate biofilms found in burns and wounds.
• Methods Development
• Laboratory methods must be validated to produce
  reliable and reproducible results.
• Methods must be cost effective at screening
  large numbers of samples (dose ranging)

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Basic MRD Experimental Setup
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Biofilm Structure

• The Biofilms tested were