Care and Use of the Microscope

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Care and Use of the Microscope
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Dos and Donts

• Replace dust cover and put up after use
• Hold with one hand on the arm and one on the base
• Only use lens paper to clean microscope lens
• Report any problems to me

• Let the cord hang off the lab table
• Drag the microscope across the lab table
• Try to disassemble the microscope

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Calculating Magnification

• Two lenses
• Objective lens
• Ocular lens (eyepiece)
• Objective lens
• Usually 3-4
• Common set scanning power (4x), low-power (10x),
  high-power (40x), and oil immersion (100x)
• Ocular lens
• (10x)
• Total Magnification objective lens magnification
  x ocular lens magnification

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Example Problem 1

• Q If the ocular lens has a power of 10x and the
  objective lens has a power of 100x, what is the
  total magnification?

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Example Problem 1

• Q If the ocular lens has a power of 10x and the
  objective lens has a power of 100x, what is the
  total magnification?
• A Multiply the ocular power times the objective
  power
• 10x 100x1000x
• Total Magnification1000x

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Example Problem 2

• Q If the ocular lens has a power of 5x and the
  objective lens has a power of 40x, what is the
  total magnification?

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Example Problem 2

• Q If the ocular lens has a power of 5x and the
  objective lens has a power of 40x, what is the
  total magnification?
• A Multiply the ocular power times the objective
  power
• 5x 40x200x
• Total Magnification200x

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Preparing a slide

• ((Didnt use this in class this time, but I
  figured Id leave it in))
• To make a temporary wet mount slide
• Get a clean slide
• Place a tiny, thin piece of the specimen on the
  slide
• Place a small drop of water over the specimen
• Place a cover slip over the specimen
• For oil emersion put a drop of oil on top of the
  cover slip

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For this Lab

• Not making a wet mount
• Remember when looking into the microscope to try
  adjusting the following to get a good view.
• Coarse focus
• Fine focus
• Iris Diaphragm
• Position of the slide
• Level of magnification