Figure 20.2 Overview of gene cloning with a bacterial plasmid, showing various uses of cloned genes

1
Figure 20.2 Overview of gene cloning with a
bacterial plasmid, showing various uses of cloned
genes
2
Figure 20.3 Using a restriction enzyme and DNA
ligase to make recombinant DNA
Restriction site
5?
3?
G A A T T C
DNA
3?
5?
C T T A A G
Restriction enzyme cutsthe sugar-phosphatebackbo
nes at each arrow
G
A A T T C
C T T A A
G
Sticky end
A A T T C
G
G
DNA fragment from another source is added. Base
pairing of sticky ends produces various
combinations.
C T T A A
Fragment from differentDNA molecule cut by
thesame restriction enzyme
G
G
A A T T
C
A A T T C
T T A A
C T T A A
C
G
G
One possible combination
DNA ligaseseals the strands.
Recombinant DNA molecule
3
Figure 12.3 Cloning a gene in a bacterial plasmid
4
Genomic libraries
5
Making an intron-lacking gene from eukaryotic
mRNA
6
Table 12.6 Some protein products of recombinant
DNA technology
7
A DNA probe tags a gene by base pairing
8
PCR
9
PCR
10
Primitive PCR machine
11
(No Transcript)
12
Applications for PCR

• DNA cloning for sequencing
• Functional analysis of genes
• Diagnosis of genetic diseases
• ID genetic fingerprints (i.e. forensics and
  paternity testing)
• Detection and diagnosis of infectious diseases
  (e.g. H1N1)

13
Gel electrophoresis of DNA
14
Lane 1 Father Lane 2 Child Lane 3
Mother The child has inherited some, but not all
of the fingerprint of each of its parents, giving
it a new and unique fingerprint.
15
Gel box and power source
16
Gel Electrophoresis Plate
17
DNA fingerprints from a murder case
18
Pharm animals
19
(No Transcript)