C. fern

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C. fern

• AP Biology

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Ferns
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Alternation of Generations
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C. Fern sporophyte
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Aseptic Technique

• ASEPTIC TECHNIQUE
• Wipe the laboratory desk counter with Clorox
  solution( 10 bleach) using paper towels.
• Use rubbing alcohol full strength from the
  supermarket (70) in a clearly marked plant
  misting bottle to spray the inside surfaces of
  your hood. Allow to dry for five ten minutes.
  The surface will be disinfected
• Wash your hands thoroughly. Do not touch
  anything before working in your hood.
• Place a beaker containing alcohol in your hood.
  Soak your spreader in the beaker. Disinfect any
  tools you are going to use before preparing your
  culture. ( scalpels, forceps, probes)
• Use sterile pipets or pipet tips.
• Keep the front of your hood partially closed.
• Do not talk or chat while you work. Do not cough
  or sneeze into the hood.
• Work slowly to keep the air still.
• Only open the top of your culture dish when you
  are ready to spread your spores on the culture or
  prepare your plant specimens for culture.
• P. Sidelsky. Revised from the Fralin
  Biotechnology Center Protocol, Virginia Tech

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C. Fern spores
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Spore germination
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Young fern gametophyte
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Mitten young gametophyte
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C. Fern - gametophyte
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Mature gametophyte
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Notch with antheridia and archegonia
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C. Fern archegonia and antheridia
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Antheridia
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Archegonia
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Flowering plants