Supplemental Figure S1. A, Suppression of OsEIN2 confers enhanced tolerance to cold, salt and drought stress. For cold stress, plants were incubated for 5 days at 3

1
B
A



C
D
OsMPK5
SalT
Fold induction
Fold induction
Hours post treatment
Hours post treatment
Supplemental Figure S1. A, Suppression of OsEIN2
confers enhanced tolerance to cold, salt and
drought stress. For cold stress, plants were
incubated for 5 days at 3 C and then returned to
greenhouse conditions for recovery. Salt stress
was triggered by immersing roots in a 250 mM NaCl
solution while drought stress was induced by
withholding water for 6 days. The levels of
abiotic stress tolerance were evaluated based on
the percentage of surviving seedlings after one
week of recovery. Asterisks indicate
statistically significant differences relative to
wild-type Dongjin (binary logistic regression
analysis a 0.05). B, ABA dose response for
germination inhibition. Germination ratio refers
to the number of germinated seeds relative to the
total number of seeds plated, with the final
number being normalized to the germination
frequency on ABA-free medium. C and D, Effect of
exogenous ABA application (0.1 mM) on transcript
accumulation of OsMPK5 and SalT in leaves of
wild-type Dongjin and OsEIN2 antisense plants. At
the indicated time points post treatment, fully
expanded fifth and sixth leaves from five plants
were harvested, converted to cDNA and subjected
to quantitative RT-PCR analysis. Gene expression
levels were normalized using actin as an internal
reference and expressed relative to the
normalized expression levels in mock-treated
control plants at 0 h. Data presented are means
( SD) of three replicates from a representative
experiment.