THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA STRAINS AND THE HUFA PROFILE DURING THE ENRICHMENT AND STARVATION

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THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA
STRAINS AND THE HUFA PROFILE DURING THE
ENRICHMENT AND STARVATION

• Xiuling Yu, Naihong Xin, Juju SunSalt Research
  Institute

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Characterization of 4 Artemia strains

• 1. Biometrics
• 2. Hatching rate
• 3. Fatty acid profile

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4 Tibetan Salt Lakes

• 1. Gaize lake (GZ)
• 2. Shuanghu Lake (SH)
• 3. Nima Lake (NM)
• 4.Yangnapengco Lake (YP).

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Table 1. Location, elevation and surface area of
4 Tibetan lakes

• Lake Location Elevation (m)
  Area (km2)
• GZ 84?05?E32?02?N 4490 86
• YP / /
  12
• SH 91?10?E31?13?N 4664 140
• NM 86?07?E30?28?N 4663 25

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Table 2 Biometrics of Artemia from 4 Tibetan
lakes
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Table 3 Hatching rate of Artemia cysts from 4
Tibetan lakes at 28 ?C (hrs)
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Table 4 HUFA content of decapsulated cysts from
4 Tibetan lakes (mg/g,D.W.)
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Enrichment and Starvation

• Two Artemia strains 1. Great Salt Lake(EG)
• 2.Laguoco Lake
  (AT)
• Three enrichment diets 1. DC Selco
• 2. DC
  Selco
• 3. A1 Selco

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Conditions

• Enrichment800ml 35ppt seawater, 27?C,
• density 300/ml(EG), 200/ml(AT),
• 0.6g/l (two ration)
• Starvation 800ml 35ppt seawater, 12 ?C,
• Density 900ml/(EG),600/ml(AT),
• The samples were taken at 6 hrs,12 hrs and 24hrs

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Table 5 The HUFA content (mg/g, D.W) of
A.franciscana (EG) and A.tibetiana after 24
hours enrichment and during starvation at 12 ?C.

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Note24E after 24 hours enrichment,
6h/12 ?C store the enriched nauplii at 12?C
for 6 hours, 12h/12 ?C store the
enriched nauplii at 12 ?C for12
hours, 24h/12 ?C store the enriched nauplii at
12 ?C for 24 hours
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The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with Super Selco
during cold storage at 12?C
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The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with A1 Selco
during cold storage at 12?C
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The decrease of DHA/EPA ratio of
A.franciscana(EG) and A.tibetiana(AT) nauplii
enriched with Super Selco during the cold storage
at 12?C
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The decrease of DHA/EPA ratio of
A.franciscana(EG) and A.tibetiana(AT) nauplii
enriched A1Selco during the cold stored at 12?C