Vitrification and Regrowth of Ribes Nigrum

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Vitrification and Regrowth of Ribes Nigrum
Student 1 Student 2 USDA-ARS NCGR Corvallis
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Question
Will Vitrification Be a Successful Method of
Preservation of the Germplasm Ribes Nigrum?
methods

• Meristem plants of Ribes Nigrum
• Mix Chemicals (Loading Solution, PVS2, and
  Sucrose rinse)
• Use Reed Vitrification Technique to place
  meristems into viles containing PVS2
• Vitrify the meristems by placing vials into
  liquid nitrogen
• Thaw vials and place plants into regrowth
  storage cells containing Ribes medium
• Place cell into controlled growth enviroment

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methods

• Vitrification
• The turning of a solid into a glass under
  extremely high temperatures or pressures.
• Liquid Nitrogen used

• Sterile Technique
• Hand placement in Laminar Flow Hood to
  Ensure Sterile Plants

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Results
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Results
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Results
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conclusions

• Regrowth in controls not always wanted
• More research is needed in vitrification
  techniques
• Vitrification can be an efficient and convenient
  method for preserving
• living tissues.
• Replant the meristems in Magenta Boxes in
  growth room
• Monitor the hardiness of the plants for at least
  six months.

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Connections

• Vitrification has many uses in the fields of
  science
• Further the advancement of cryobiology
• Efficient and convenient method of nuclear waste
  disposal and
• preservation of internal organs
  
• Useful to conserve plant tissues for
  experimentation and preservation