Efficacy of Contact Lens Disinfecting Solutions Against Pseudomonas Aeruginosa, Staphylococcus Aureu

1
Efficacy of Contact Lens Disinfecting Solutions
Against Pseudomonas Aeruginosa, Staphylococcus
Aureus, Fusarium Solani

• Manuj Kapur, MD
• Charlise Gunderson, MD
• Department of Ophthalmology and Visual Sciences
• University of Texas Medical Branch at Galveston

2
Commercial Support Disclosure

• Manuj Kapur does not have any financial
  arrangements or affiliations with any commercial
  organizations.

3
Compliance with Contact Lens Care Guidelines

• 35 million contact lens (CL) wearers in USA
• 25 millions SCLs
• Rate of SCLs related infections inversely
  proportional to compliance with contact lens care
  guidelines, specified by soln man.
• Rate of compliance was 72 for new SCLs wearers
• Rate of compliance was 38 for long-term SCLs
  wearers

Chun MW, Weissman BA. Compliance in contact lens
care. Am J Optom Physiol Opt 1987 64 274-276.
4
Non-compliance with contact lens care guidelines
Staphylococcus Aureus
Pseudomonas Aeruginosa
20/20
CF at 1 feet
20/20
20/400
5
Minimizing Contact Lens Related Infections

• Patient Education !
• Patient Education !
• Patient Education !
• Recommending the best contact lens disinfecting
  solution

6
How do you recommend a contact lens solution?

• Criteria in selecting a contact lens (CL)
  solution
• Highest disinfectivity
• Lowest corneal toxicity
• Low cost
• Sample availability
• Direct consumer advertising
• Media influence like reports of Fusarium
  contamination in CL solutions

7
Study Design

• Determine which of the commercially available
  contact lens disinfection solutions meets the ISO
  14729 guidelines of 3-log reduction during
  recommended soak time for bacteria, and 1-log
  reduction for fungi species

8
Tested Contact Lens Solutions
9
Materials and Methods

• Test Solutions
• Opti-Free Express with ALDOX
• ReNu Multi-Plus
• 0.9 Normal saline solution

10
Hypothesis

• ReNu Multi-Plus and Opti-Free Express with
  ALDOX will meet ISO 14729 standard of 3-log
  reduction at their respective soak times with
  respect to Pseudomonas Aeruginosa and Staph
  Aureus. However, ReNu Multi-Plus will not
  meet ISO standards for fungi, especially Fusarium
  Solani.

11
Materials and Methods

• Test Organisms
• Pseudomonas Aeruginosa ATCC 27583
• Staphylococcus Aureus ATCC 29213
• Fusarium Solani
• Test Procedure
• 0.1 mL of 1 X 108 CFU/mL of inoculum
• 9.9 mL of each test solution in glass tube
• Each isolate mixture sampled at 25 C for
  specified times of 4, 6, 9, and 24 hours
• 1 microliter removed and plated TSA blood agar
  plate
• Plates incubated for 24 hrs at 37 C
• Experiment performed as triplicate

12
Results for Opti-Free Express
3.2 reduction
7
7
7
2.7 reduction
6.0 reduction
13
Results for ReNu Multi-Plus
3.2
4.2
7
2.6 reduction
6.0 reduction
6.0 reduction
14
Conclusions

• ReNu Multi-Plus Opti-Free with ALDOX met ISO
  standards of 3-log reduction for Pseudomonas
  aeruginosa and Staph aureus, and achieved a 1-log
  reduction for Fusarium solani. Therefore, both
  solutions met the ISO standards for these stand
  alone contact lens solutions.

15
References

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  14729 Ophthalmic optics Contact lens care
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