Amylase is an enzyme capable of breaking a starch polysaccharide into monosaccharide

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Amylase is an enzyme capable of breaking a starch
polysaccharide into monosaccharides. Lugols
solution (contains iodine) can be used to detect
the presence of starch. A positive test is dark
blue. Benedicts solution (contains Cu) can be
used to detect the presence of monosaccharides.
The Cu reacts with the aldehyde group and forms
Cuprous oxide. Cuprous oxide precipitates out of
solution as a green to brownish red color.
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Starch amylase
Starch only
Incubate at 37?C for at least 30 minutes
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Lugols
Lugols
Benedicts
Benedicts
or - ?
or - ?
or - ?
or - ?
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pH?
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Lipase is an enzyme that can digest lipids
(triglycerides). The digestion of triglycerides
results in the release of fatty acids, which
causes a decrease in pH. Remember that pH
decreases as a solution becomes more
acidic. Phenol red is red at pH gt 7 and is
yellow at pH lt 7. We can use the color of
phenol red as an indicator of pH thus lipid
digestion. We can add bicarbonate to milk to
increase pH so when phenol red is added it is
pinkish.
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Page 466
Which one will change the fastest? Will
temperature affect the rate of change?
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Proteins are polymers of amino acid monomerss
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The enzyme pepsin breaks down proteins (polymer)
into amino acids (monomers). This enzyme works
best in a low pH environment. Biurets solution
turns pink in the presence of amino acids, so it
can be used to test for protein digestion (Ill
do this as a demonstration). Ninhydride is
purple in the presence of protein. The more
intense the color the more protein present.
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