An%20employment%20of%20flow%20cytometry%20in%20the%20biosystematics%20of%20the%20genus%20Galeobdolon

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An employment of flow cytometry in the
biosystematics of the genus Galeobdolon

• Radka Rosenbaumová

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Genus Galeobdolon
x 9 2x 18 4x 36 3x 27
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Genus Galeobdolon
G. luteum
G. flavidum
2n 2x 18
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Genus Galeobdolon
G. montanum
2n 4x 36
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Genus Galeobdolon
G. argentatum
2n 4x 36
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Genus Galeobdolon
G. montanum
G. luteum
G. endtmannii
4x
2x
2n 2x 18
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Genus Galeobdolon
Distribution
G. argentatum
G. endtmannii
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Ploidy level estimation
Standard Glycine max cv. Polanka, (2C 2.5 pg)
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Ploidy level estimation
Triploids
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Ploidy level estimation
Triploids
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Ploidy level estimation
Sympatric occurence
12 G. luteum G. montanum G. argentatum
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Ploidy level estimation
G. endtmannii
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Ploidy level estimation
G. endtmannii
G. luteum
G. montanum
4x
2x
4x
2x
2x 4x
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Nuclear genome size determination
Species 2C DNA content SE (pg) 1C DNA content (pg / Mbp) Genome size (pg)
G. luteum 3.27 0.002 1.63 / 1597 1.63
G. endtmannii 3.28 0.02 1.64 / 1607 1.64
G. flavidum 3.41 0.02 1.70 / 1666 1.70
G. montanum 6.33 0.04 3.17 / 3107 1.58
G. argentatum 6.36 0.04 3.18 / 3116 1.59
G. montanum 6.59 0.04 3.29 / 3224 1.65
G. endtmannii 6.41 0.03 3.20 / 3136 1.60
Triploids 4.85 0.04 2.43 / 2381 1.62
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Nuclear genome size determination
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Nuclear genome size determination
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Nuclear genome size determination
G. luteum / G. flavidum
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Nuclear genome size determination
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Nuclear genome size determination
G. argentatum X G. montanum

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Nuclear genome size determination
G. argentatum X G. montanum
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Conclusions
3x Sympatric occurence G. endtmannii G.
flavidum G. montanum X G. argentatum
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An employment of flow cytometry in the
biosystematics of the genus Galeobdolon