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Effective Phage Therapy is Associated with Normalization on of Cytokine Production by Blood Cell Cul

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Title: Effective Phage Therapy is Associated with Normalization on of Cytokine Production by Blood Cell Cul


1
Effective Phage Therapy is Associated with
Normalization on of Cytokine Production by Blood
Cell Cultures
  • Beata Weber-Dabrowska, Michal Zimecki and Marian
    Mulczyk
  • Presentation by
  • Marc Palazzo

2
History
  • Felix DHerelle discovered bacteriophage
  • First licensed phage preparation in 1930s
  • Luria Delbruck
  • Kantoch et al. binding to cancer cells (1940s)
  • Chachoua and Dabrowska binding of phage to
    immune cells

3
Delmont Labs
Produces phage lysate for use by Vets on animals
4
Vocabulary
  • Tumour necrosis factor (TNF) alpha a protein
    which has a wide range of pro inflammatory
    actions. TNF can kill cancer cells in vivio and
    in vitro.
  • Interleukin-6 Cytokine that stimulates growth
    and differentiation of human B-cells.
  • Integrins Cell surface proteins involved in
    binding to extracellular matix components.
  • Cytokine Signaling molecules perform cell-cell
    communication.

5
Hypothesis
When phage therapeutics are used is the
resulting effect only caused by the lyses of
bacteria, or perhaps is there an interaction
between virus and the immune system?
6
Cytokines to Study
IL-6 Initiates immune response TNF-alpha
Helps trigger immune response to pathogens
Lets find out how phage therapy affects cytokine
production with and without LPS
7
Materials
  • 51 sick patients
  • Categorized patients on own immune status
  • Low, moderate, high cytokine levels
  • 8 healthy people
  • Bacteriophages (Unknown type) were administered
    locally
  • Peripheral blood taken and assayed
  • At 0, 7, and 21 days

8
Methods
  • IL-6 100uL of serially diluted plasma were put
    in 96-well plates along with 7TD1 indicator
    cells. After culturing for 72 hours the OD was
    taken
  • TNFa Indicator WEHI was mixed with diluted
    plasma in 96-well plates. After overnight
    incubation the OD was taken
  • Cell death/proliferation Overnight incubation at
    37 degrees the OD was taken using wavelength of
    550nm.

9
Figure 1
With Phage Therapy
10
Figure 2 3
11
Figure 4
12
Table 1
13
Just a Phage Im Going Through
Data shows there is not only stimulation of
immune cells, but regulation of them. In Vitro
blood cells to produce cytokines when
stimulated differed. Effects on IL-6 were less
evident although regulation occurred Study
showed if cells hyporeactive to TNF production
then phage therapy was unsuccessful
14
Unknown what actually causes immunoregulation Mayb
e Result of recovery from infection given normal
cytokine production Bacteriophages are actually
giving immunoregulation by their
interactions Or bacterial debris with the phage
lysate gives results
Article did show regulation of cytokine
production during phage therapy
15
What it all means
The World is a phage
Coupled with other articles other info
resources Good Science
Phages are able to amplify and strengthen some
WBCs
In Vivo phages can induce fever and hormonal
modulation
In Vitro shown to increase cellular
multiplication and division (Chachoua and S.L.
Wenger 1978)
16
Be Creative
Data suggests there is virus to membrane
interaction KGC (Lys-Gly-Asp) sequence on the
T4 phage may utilize Beta 3 integrin on the host
cell Perhaps interference may be able to slow
infection down with HIV (both adsorbing to
similar integrins)
Cancer cells also have Beta 3 Integrin Is this
a cancer therapy?
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