Removal of indicator bacteria, human enteric viruses, Giardia cysts, and Cryptosporidium oocysts at - PowerPoint PPT Presentation

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Removal of indicator bacteria, human enteric viruses, Giardia cysts, and Cryptosporidium oocysts at

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Removal of indicator bacteria, human enteric. viruses, Giardia cysts, and ... The plant was not efficient at removing indicator bacteria and pathogens tested ... – PowerPoint PPT presentation

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Title: Removal of indicator bacteria, human enteric viruses, Giardia cysts, and Cryptosporidium oocysts at


1
Removal of indicator bacteria, human
entericviruses, Giardia cysts, and
Cryptosporidiumoocysts at a large wastewater
primary treatmentfacility
  • Presenter Jonas Lindkvist
  • Topic Pathogen removal in wastewater treatment
    plants
  • By Pierre Payment, Robert Plante, Patrick
    Cejka
  • Source Science Direct
  • Published February 26, 2001

2
Introduction
  • Montréals waste water treatment plant
  • Largest waste water treatment plant in Canada
  • Treat waste water from over one million people
    including the industry of Montréal
  • Treats a volume of water representing 50 of the
    waste waters treated in Québec.
  • Can treat 2 billion U.S gallons per day

3
Picture of Montréals waste water treatment plant
4
  • A examination have been done of the plants
    ability to remove pathogenic bacteria from
    wastewater.
  • 6 month occurrence study
  • May - October
  • Samples collected 4 times per week

5
Examined pathogens (P) and indicator bacteria (I)
  • Human enteric viruses (P)
  • Giardia lamblia (P)
  • Cryptosporidium parvum (P)
  • Clostridium perfringens (P)
  • Fecal coliforms (I)
  • E.coli (I)
  • Fecal streptococci (I)

6
Human enteric viruses
  • No treatment for diseases caused by these
    viruses.
  • Poliovirus This disease have a effective
    vaccine

7
Giardia lamblia
  • A parasite that is transmitted through water
    infected with feces
  • Colonize small intestine in humans
  • Symptom - stomach trouble
  • - diarrhea

8
Cryptosporidium parvum
  • Protozoa A single-celled animal
  • Cause - intestinal infection with severe
    diarrhea
  • C. Parvum lining the intestinal tract

9
Clostridium perfringens
  • Occurs in the intestines of humans
  • Produce toxin that cause
  • - Death (rare)
  • - severe illness
  • - Intense pain
  • - Diarrhea

10
E.coli
  • The most common bacteria in humans
    gastrointestinal tract
  • Often no symptoms
  • Sometimes symptoms as
  • - urinary infection
  • - diarrhea

11
Fecal streptococci and Fecal coliforms
  • Indicators of recent fecal pollution because they
    are commonly found in human and animal feces
  • Indicate the possible presence of pathogenic
    (disease-causing) bacteria, viruses, and
    protozoans that also live in human and animal
    digestive systems

12
Methods and experimental design
  • 3 liters of untreated and treated water was
    collected four times per week between May and
    october
  • 2 liters were used for the virus and the
    pathogens. 1 liter for bacteriological analysis.

13
Methods and experimental design
  • Used membrane filtration methods to dilute them.
  • Concentrated water were centrifuged at
  • 3000 x g for 30 min at 4?C

14
Methods and experimental design
  • Detection of parasites and viruses
  • They did not distinguish between dead and live
    parasites
  • Used immunoflourescence to count the cells.

15
Table 1. Indicator bacteria and pathogens at the
Montreal waste water treatment plant before
and after treatment
16
Presented results
  • Human enteric viruses
  • - no removal observed
  • Giardia lamblia
  • - 76 removed from treatment
  • Cryptosporidium parvum
  • - 26 removed from treatment
  • Clostridium perfringens
  • - 51 removed from treatment
  • Fecal coliforms
  • - 25 removed from treatment

17
Presented results
  • E.coli
  • 12 removed from treatment
  • Fecal streptococci
  • 29 removed from treatment

18
Conclusions
  • The plant was not efficient at removing indicator
    bacteria and pathogens tested
  • To protect public health additional treatment and
    disinfection is recommended.

19
Critique
  • This report should have been worked on more
    before it was released. They should have
    explained more about for example methods used,
    and also had more headlines.
  • Methods and equipment were not described well.
  • Hard to see results from diagrams
  • Dividing up article was done in a bad way with no
    structure
  • Used strange background information in some
    parts.

20
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