DETECTION OF DENATURATION OF PROTEIN AT LOW TEMPERATURE USING FTIR

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DETECTION OF DENATURATION OF PROTEIN AT LOW
TEMPERATURE USING FTIR

• BY VIKAS W. KAPOOR
• CHEMICAL ENGINEERING DEPARTMENT
• SYRACUSE UNIVERSITY
• SYRACUSE

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CONTENT

• Denaturation of protein
• Denaturation occurring at lower temperature
• Its detection using FTIR
• Results and conclusion

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Denaturation of proteins

• What is denaturation of protein?
• It is the change taking place in protein
  structure in such a way that they are no more
  functional. These changes are mainly dependent on
  their secondary and tertiary structures.

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Structue of protein
a. Primary Structure b. Secondary Structure c.
Tertiary Structure d. Quaternary Structure
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Effect of denaturation on properties of protein.

• Decrease in solubility
• Altered water binding capacity
• Loss of biological activity
• Improved digestibility
• Increased intrinsic viscosity
• Inability to crystallize

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Techniques to detect protein denaturation.

• Fourier Transform Infrared Spectroscopy(FTIR)
• Differential Scanning Calorimetry(DSC)
• Circular Dichroism(CD)
• Birefringence
• Optical opacity

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Denaturation at lower temprature

• Need of this study
• Reasons of denaturation at lower temperature on
  proteins
• Study of AT - 1 Dunning prostrate tumor cells
  done at control condition(normal condition),
  -20C and - 80C by using FTIR to see the
  denaturation of protein occurring at freezing
  temperatures.
• It was observed using FTIR that protein
  denaturation was observed significant at - 80C
  than -20C

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Fourier Transform Infrared Spectroscopy
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RESULTS
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