SwedenVietnam Research Cooperation Programme SubProgram Biotechnology

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Sweden-Vietnam Research Cooperation
ProgrammeSub-Program Biotechnology

• Presented by
• Dr. Truong Nam Hai
• Coordinator of Subprogram Biotechnology
• Hanoi, 18-19 May 2005

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The activity of Sub-program Biotechnology in 2004

• Two workshops were organized for people of five
  projects
• The annual review meeting for 2004 activity and
  proposal plan for 2005

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Workshop Method in protein purification

• Implementing organization Institute of
  Biotechnology (IBT)
• Content Introduction on techniques to separate
  biochemical compounds used in protein
  purification as HPLC, FPLC, gel filtration, ion
  exchange chromatography
• Place Quang Ninh Province, Viet Nam
• Duration From 3-7 October 2004.
• Lecturer Prof. Dr. Jan-Christer Janson
• Center for Surface
  Biotechnology, Uppsala, Sweden.
• Participants gt30 persons

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Workshop on The training methods in fungal
identification

• Implementing organization Institute of
  Agricultural Genetic and Biotechnology (IGAB)
• Objective
• - To developing the sensitive, reliable and
  cheap methods of fungal identify for
  microorganisms management
• - Fieldwork Sample collection in Yen Bai
  province and practical work as ELISA
  technique at IGAB, Hanoi.
• Duration From 13-17 December 2004.
• Lecturer Assoc. Prof. Kasem Soytong
• King Mongkuts Institute of
  Technology, Thailand
• Participants gt30 persons

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Annual review meeting for 2004 activity and
proposal plan for 2005

• Implementing organization Institute of
  Biotechnology
• Purpose Report on research activity of five
  projects and propose activity plan of Sub-program
  Biotechnology for 2005
• Place Quang Ninh Province, Viet Nam
• Duration From 23-24 December 2004.
• Chairman Dr Truong Nam Hai
• Participants gt30 persons from five projects of
  Subprogram BT

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Financial Report

• Budget for 2004 SEK 250,000
• Detailed balance sheet of workshops
• Workshop Methods in protein purification
  VND 79,848,493
• The training Methods in fungal identification
  VND 83,013,540
• Annual review meeting and proposal plan for
  2005 VND45,989,449
• Total budget for Subprogram activity in 2004 VND
  208,851,500
• 
  SEK 104,425.75

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Activity plan of Subprogram biotechnology in 2005

• 1. Workshop on Enzyme Technology and
  Biosensor
• 2. Workshop on Improvement of skills for
  writing scientific research proposals
• 3. Workshop on Plant gene transformation and
  molecular Biology
• 4. Workshop on Improvement of skills for
  writing an international paper
• 5. Annual review meeting and proposal for
  2006 plan

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Workshop on Enzyme Technology and Biosensor

• Implementing organization Hanoi University of
  Education (HUE)
• Workshop provides for participants the knowledge
  on enzyme technology and biosensor in general,
  especially the update knowledge on enzyme
  producing by immobilizing cells and enzymes
• Place Agro-Biology Faculty, HUE, Hanoi
• Duration From 28 Feb -3 Mar 2005.
• Lecturers Prof. Bo Mittiason and Prof. Rajni
  Hatti-Kaul, Centre for Chemical Engineering, Lund
  University, Sweden
• Participants gt30 persons

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research Activity of five projects in 2004
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• BT1 - Forest Science Institute of Vietnam (FSIV)
• Research Centre for Forest Tree Improvement
  (RCFTI)

USE OF GENETIC DIVERSITY AND BIOTECHNOLOGICAL
ADVANCES IN FOREST TREE IMPROVEMENT
RESEARCH Project Director Dr. Ha Huy Thinh
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• RESEARCH RESULTS
• Breeding and trial establishment
• Data collection were done in progeny trials of P.
  kesiya, Eucalypts, Acacias, Melaleucas. 20
  families were collected to establish a
  demonstration plantation in Lamdong.
• Developed grafting techniques for 30 clones of P.
  caribaea , P. massoniana and 30 clones of E.
  urophylla
• Study on flowering and control pollination
• Evaluated the flowering and seed production of P.
  caribaea
• Performed pollen collection and germination test
  for P. merkusii and P. caribaea. Studied on
  effect of moisture content and storage condition.
• Controlled pollination between P. massoniana, P.
  merkusii and P. caribaea, hybrid seed was
  collected

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• Study on Biotechnology
• Creation of polyploidy, embryogenesis and study
  on effect of physical aging and propagation
  technique on the rooting
• Using micro-satellite markers in evaluation of
  genetic diversity and out cross-link rate in seed
  orchards
• Mating design and control pollination
• Published 5 papers in 2004
• Training
• Supported for 3 Ph.D students in Sweden for
  field survey, data collection (3 months for each)
  and 1 MSc. student in Vietnam

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• BT2- Institute of Agricultural Genetics
  Biotechnology (IAG B)

APPLICATION OF BIOTECHNOLOGY IN RESEARCH AND
DEVELOPMENT OF NON-CHEMICAL CONTROL OF COFFEE
PRODUCTION IN VIETNAM Project Director Dr. Le
Thi Anh Hong
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• Research content using various methods to
  identify pathogen fungi isolated from different
  coffee production areas in Vietnam

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• Training
• 1 Ph.D student and 1 MSc. in Sweden
• 2 MSc. students in Vietnam
• BT2 organized the training course Methods in
  fungal identification for Sub-program in Dec.
  2004

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• BT3 - Institute of biotechnology (IBT)

production of recombinant proteins for
agricultural and medical use Project Director
Dr Truong Nam Hai
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• Research results
• Part 1 Improving of enzyme activity of amylase

• Created a library of amylase mutant using
  error-prone PCR and DNA shuffling.
• Selected one amylase mutant with one amino acid
  substituted and the amylase activity increased
  twice compare to that of wild type.

• Part 2 Production of recombinant proteins of
  Salmonella for vaccine and diagnostic
• Cloned antigenic determinants of Salmonella as
  fliC1/2/3 fljB1/2, gm1/2/3 and sef14 genes of S.
  typhimurium and S. enteritidis
• Expressed fljB, fljB2, gm1, gm2 genes encoding
  for flagella antigen of S. typhimurium and S.
  enteritidis in E. coli BL21.

fljB2
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• Part 3 Cloning and expression of Bacillus
  collagenase
• Constructed a genomic library of B. subtilis
  FS-2 in E. coli DH5?
• Selected one recombinant strain having
  collagenase activity from a genomic library
• Determined the sequence of cloned gene and its
  restriction enzyme map
• Cloning and expression of collagenase gene in E.
  coli DH5?

Negative control pUC18
Collagenase activity of the selected recombinant
strain

• Training
• 2 Ph.D in Sweden (2005) and 2 MSc. students in
  Vietnam (2004)
• BT3 organized 2 workshops for Subprogram
• Published 2 papers in 2004

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BT4 - Nong lam university in ho chi minh (NLU)
USING BIOTECHNOLOGY IN IMPROVEMENT OF SESAME AND
CRABME Project Director Prof. Dr Bui Cach Tuyen
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• Research activity in 2004
• Set up a plan for sampling Sesame accession
  around Vietnam including North Vietnam, the
  High-land, Southeast and the Mekong delta.
• Training two Ph.D students in Sweden.
• BT4 agreed to organize the workshop Plan gene
  transformation and molecular Biology in 2005

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BT5 - Hanoi University of Education (HUE)
Study on using agriculture by-products for
producing microbial enzyme-feed used for poultry
and pig husbandry in Vietnam Project Director
Dr. Mai Thi Hang
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• Research results

Screening and improving enzyme productions of the
selected clones by conventional methods
Improve enzyme productions of the selected
strains by traditional methods as studying on the
effect of UV, chemical mutation, pH, temperature
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Screening for probiotic microorganismsOver 300
strains were screened in which 5 strains of
lactic bacteria produce probiotic and 4 yeast
strains were amino acid producersStudying on
effect of the selected enzyme on growing of the
weaning swine at lab modelSupplemention phytase
and some enzymes improved growth performance and
feed efficiency in growing pig

• Training
• 2 Ph.D, 4 MSc. students and 1 technician
• BT5 organized the workshop Enzyme Technology
  and Biosensor in Mar. 2005 for Sub-program.

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Comments

• We need the official appointment documents for
  Subprograms Coordinator (Audit comment)
• We need finance support from SIDA for projects
  activity in time (Audit comment).
• We have problem in VAT refund. We hope to solve
  it in 2005.

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• Thank you