The Model Experiment

1
The Model Experiment
2
A Complex Behavior
Modularize
Module A
Module B
Module C
Module D
Behavior orthologs
Well studied non-model example
Find appropriate model system?
Yes
No
Analyze
Literature
Search
Mutants
Literature
Study
Evaluate
Candidate Genes
Lab Assays
Candidate Genes
Experimental Methods
Experimental Methods
Generate Hypotheses and design experiments
3
Example Insect Behavior
Module D
Module A
Module B
Module C
Ortholog 3
Ortholog 4
Ortholog 2
Fly, Mouse
Ortholog 1
Fly
Termite
Fly
Fly, Mouse
Literature
Mutants
Literature
Candidate Genes
Lab Assays
Experimental Methods
Candidate Genes
Experimental Methods
Generate Hypotheses and design experiments
4
A Complex Behavior
Analyze gene expression
Variant A
Variant B
Variant C
Variant D
Gene expression profiles
Ortholog analysis
Analyze
Look for
Co-regulation
Meta-analyze
Study
Functional annotation
Correlated expression
Pathways
Generate Hypotheses and design experiments
5
Example Insect Behavior
Microarray analysis
Population B
Population A
Population C
Genes with different expression profiles between
populations
Analyze
Fly and mouse orthologs
Look for
Co-regulation
Meta-analyze
Study
Functional annotation
Correlated expression
Pathways
Generate Hypotheses and design experiments
6
The Fantastic BeeSpace
Other entities
Type query
Entity Identifier
Different PnPs
Assay Engineer
Behavior
Candidate Gene Bank
Mutant Base
Module Maker
Hypothesis Generator
Tuned by user feedback
Name and number the modules, describe each module
Experiment Builder
Ortholog Search Engine
Model system Finder
User select focal model system
User approved