Characterization of Enterococci Found in Marine and Intertidal Sediments and Coastal Water in South

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 Characterization of Enterococci Found in Marine
and Intertidal Sediments and Coastal Water in
Southern California D.M. Ferguson, D.F. Moore,
M.A. Getrich and M.H. Zhowandai Orange County
Public Health Laboratory, Newport Beach, CA, USA
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Total Coliforms (Group)
Fecal Coliforms
Enterococcus (Genus)
Escherichia (Genus)
coli (Species)
faecalis (Species)
(Strain) 0157H7 (Pathogen)
(Strain) Garden Variety
(Strain) Pathogen
(Strain) Garden Variety
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Speciating Bacteria
Sample Collection
Water
Sediment
Pick single colony
Membrane Filtration
Sediment Sonication
Purify
Identification API conventional tests
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Baby Beach Marine Water
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Baby Beach Sediment
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Gull Stools from Baby Beach
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Pulsed-Field Gel Electrophoresis (PFGE)
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Conclusions

• Higher densities of enterococci in intertidal
  sediments as compared to beach water
• Enterococci species distribution was similar in
  beach water and intertidal sediments
• Species distribution similar in water samples
  passing and failing standards
• Findings suggest constant loading of stable
  enterococci population to beach water from a
  continual source such as storm drain runoff
  and/or sediments