Two-Photon Microscopy with Wavelength Switchable Fiber Laser Excitation Jay Unruh, Shane Price, Roque Gagliano, Lisa Stehno-Bittel, Carey Johnson,a Rongqing Hui ([e-mail])

1
Two-Photon Microscopy with Wavelength Switchable
Fiber Laser ExcitationJay Unruh, Shane Price,
Roque Gagliano, Lisa Stehno-Bittel, Carey
Johnson,a Rongqing Hui (rhui_at_ku.edu)
System configuration
Nonlinear wavelength shift with PCF
Multi-wavelength two-photon cell images
Two-photon image of developing vascularture in
quail embryos
A) Cells stained with dapi which the laser was
able to excite at 810nm. B) Same section stained
for fibrillin and excited at 1050nm. Both images
false-colored. Size of each picture 50mm x 50mm.