Problem Analysis

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Problem Analysis

• Subspecies Definition
• Falsifiable Hypotheses
• Discriminating Test Criteria

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Hypotheses

• Proposed subspecies as hypotheses
• Subspecies should be distinguishable from each
  other
• Groups within subspecies should not be
  distinguishable

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Tests

• Cranial Morphometric Analyses
• Distinguishable by shape (vs size)
• Linear Discriminant Analysis
• gt 90 _at_ jackknifed posterior P gt 0.95
• Eliminated age effects
• Genetic Analyses
• mtDNA (near) reciprocal monophyly
• (ESU Moritz 1994)

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mtDNA control region sequences (515bp)

• Eastern Sierra (ES)
• Sierra Nevada 18
• Inyo Mountains 18
• Hunter Mountain 17
• Last Chance Range 16
• White Mountains 16
• Mojave Desert (MD)
• 25 mountain ranges 394 (Clint Epps)

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Mt DNA results

• 32 different haplotypes
• 15 haplotypes gt 3 bases different
• 3 ES haplotypes notably more different from rest
  (Sierra, Inyo Mtns., Hunter Mt.)

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Nuclear DNA

• Data for 12 microsatellite loci
• 5 Sierra Nevada (212) 1980s Baxter (38)
• White Mountains (16)
• Inyo Mountains (31)
• Hunter Mountain (20)
• Last Chance Range (21)
• 25 Mojave Desert populations (Clint) 394

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Summary

• Morphometric/mtDNA results concordant
• Sierra bighorn at least as different from desert
  bighorn as Rocky Mountain bighorn
• The more we test, the more unique
• Sierra bighorn bottlenecked lower diversity
• Diversity not an issue
• Work in progress