Control of Autoimmune Diabetes in NOD mice by GAD Expression or Suppression in Beta Cells

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Control of Autoimmune Diabetes in NOD mice by GAD
Expression or Suppression in Beta Cells

• Yoon, J.W., C.S. Yoon, H.W. Lim, Q.Q. Huang, Y.
  Kang, K.H. Pyun, K. Hirasawa, R.S. Sherwin, and
  H.S. Jun

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Rational for Figure 1A

• NOD mice that have the antisense GAD transgene
  for both forms of the isoform or rat GAD cDNA
• Control is the RIP

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Data for Figure 1A
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Rational for Figure 1B-C

• Which animals are transgenic
• In 1C it shows which mice are high, medium or low

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Data for 1B-C
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Conclusion for 1B-C

• The lanes that have the most pronounced bands are
  considered to be H-AS-GAD-NOD
• The lanes with medium band visibility are
  considered to be M-AS-GAD-NOD
• Those with low or almost no band visibility are
  labeled L-AS-GAD-NOD

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Rational for 1D-E

• Use of Northern blot to identify which mice are
  transgenic H-, M-, or Low (AS-GAD65.67)
• Islet cells are more specific to suppression of
  antisense GAD than brain cells

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Data for Figure 1D-E
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Conclusion for 1D-E

• H-AS-GAD-NOD has no GAD production in islet
  cells, however there is GAD produced in brain
  cells
• M-AS-GAD-NOD has little GAD production in islet
  cells and GAD produced in brain cells
• L-AS-GAD-NOD and (-) produces GAD in both islet
  cells and brain

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Rational for Figure 1F

• Is the GAD suppression of each group apparent in
  actual staining of the islet cells

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Data for 1F
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Conclusion for 1F

• H-AS-GAD-NOD mice have no GAD expression
• L-AS-GAD-NOD and M-AS-GAD-NOD mice have GAD
  expression
• However each AS line have no difference in the
  amount of pancreatic insulin concentration