M184V confers

1
M184V confers High -level resistance to 3TC
2
M184V Diminishes Chain-Termination
WT RT
M184V
Full- Length DNA
Chain- termination
Primer
3
HIV Resistance to 3TC
4
AZT-associated Mutations are not always located
in the vicinity of the dNTP binding site
5
Classical AZT-associated mutations do not reduce
rates of incorporation of AZT-MP
WT RT
41 / 67 / 210 /215
Full- Length DNA
Chain- termination
6
HIV Resistance to AZT
AZT-TP
PPi
RT. DNA-AZT-MP
RT.DNA
7
Drug Hypersusceptibility and Resensitization
AZT-TP
PPi
RT. DNA-AZT-MP
RT.DNA
AZT-resistant RT
Wild-type RT
3TC-resistant RT (M184V)