Enzyme Kinetics II - PowerPoint PPT Presentation

About This Presentation
Title:

Enzyme Kinetics II

Description:

Although a phenomenological description can be ... Ping-Pong Reactions. Group transfer reactions. One or more products released before all substrates added ... – PowerPoint PPT presentation

Number of Views:25
Avg rating:3.0/5.0
Slides: 23
Provided by: william203
Learn more at: http://nsmn1.uh.edu
Category:
Tags: enzyme | kinetics | ping | pong

less

Transcript and Presenter's Notes

Title: Enzyme Kinetics II


1
Enzyme Kinetics II
  • 10/16/2008

2
Kinetic data cannot unambiguously establish a
reaction mechanism.
Although a phenomenological description can be
obtained the nature of the reaction intermediates
remain indeterminate and other independent
measurements are needed.
3
Reaction MechanismsA Sequential Reactions
  • All substrates must combine with enzyme before
    reaction can occur

4
Bisubstrate reactions
5
Random Bisubstrate Reactions
6
Ping-Pong Reactions
  • Group transfer reactions
  • One or more products released before all
    substrates added

7
Inhibition kinetics
  • There are three types of inhibition kinetics
    competitive,
  • mixed and uncompetitive.
  • Competitive- Where the inhibitor competes with
    the substrate.

8
Competitive Inhibition
9
(No Transcript)
10
(No Transcript)
11
HIV protease inhibitors
12
Competitive Inhibition Lineweaver-Burke Plot
13
Uncompetitive Inhibition
14
Uncompetitive Inhibition Lineweaver-Burke Plot
15
Mixed inhibition
16
(No Transcript)
17
Mixed inhibition is when the inhibitor binds to
the enzyme at a location distinct from the
substrate binding site. The binding of the
inhibitor will either alter the KM or Vmax or
both.
18
(No Transcript)
19
The effect of pH on kinetic parameters
20
(No Transcript)
21
(No Transcript)
22
Lecture 17Tuesday 10/21/08Enzyme Kinetics /
Exam II Minireview
Write a Comment
User Comments (0)
About PowerShow.com