Cell Sorting For The Isolation of Stem Cells For Their Use in Tissue Engineering

1
Cell Sorting For The Isolation of Stem Cells For
Their Use in Tissue Engineering
UK Centre for Tissue Engineering
R.V.Walker, J.A.Hunt, N.P.RhodesUKCTE,
Department of Clinical Engineering, University of
Liverpool

• Introduction Stem cells are important in
  Tissue Engineering as they can be promoted into
  different lineages by addition of growth factors
  and variation of culture conditions. As they are
  often found in small percentages in a mixed cell
  population, their isolation and purification is
  essential. This can be achieved using cell
  sorting.

Results of Sort


Haematopoietic progenitor stem cells can be found
in
Nozzle
Laser beam
Forward Scatter Photodiode
Click to find out more
Pulse of Charge Applied
Charged Deflection Plates
Application of this technique This technique is
being used for the creation of tissue engineered
heart valves. Click to find out more

-
Cord blood
Peripheral blood

Foetal Liver
Bone Marrow
Waste Catcher

-

-
Sort Receptacle
Click to find out more
These stem cells are positive for markers CD34
CD45


Conclusion It is possible to sort rare
populations of stem cells from peripheral blood
using high speed cell sorting. Stem cells appear
to hold the key to Tissue Engineering and
therefore their isolation into a single, pure
population is very important.
CD34
CD45
CD34CD45
MoFlo cell sorter Capable of sorting
100,000 cells/second at up to 95 purity. CLICK
TO START A SORT
Click to find out more
MoFlo Diagrams adapted from