Supplemental Figure 4. Quantitative FRAP analysis of control proteins. The analyses have been carried out in transiently transformed tobacco BY-2 cell protoplasts (p) and/or in root cells of stably transformed Arabidopsis seedlings (s) expressing the - PowerPoint PPT Presentation

1 / 1
About This Presentation
Title:

Supplemental Figure 4. Quantitative FRAP analysis of control proteins. The analyses have been carried out in transiently transformed tobacco BY-2 cell protoplasts (p) and/or in root cells of stably transformed Arabidopsis seedlings (s) expressing the

Description:

... of the nucleus is indicated by blue x, indicating the fading during the experiment. The background fluorescence measured outside the nucleus is indicated by yellow ... – PowerPoint PPT presentation

Number of Views:39
Avg rating:3.0/5.0

less

Transcript and Presenter's Notes

Title: Supplemental Figure 4. Quantitative FRAP analysis of control proteins. The analyses have been carried out in transiently transformed tobacco BY-2 cell protoplasts (p) and/or in root cells of stably transformed Arabidopsis seedlings (s) expressing the


1
Supplemental Figure 4. Quantitative FRAP analysis
of control proteins. The analyses have been
carried out in transiently transformed tobacco
BY-2 cell protoplasts (p) and/or in root cells of
stably transformed Arabidopsis seedlings (s)
expressing the indicated GFP/YFP fusion proteins.
The graph depicts the entire time course of the
experiment including pre-bleach, bleach and
recovery phase. Time 0s is the begin of the
recovery phase after bleaching. Normalized FRAP
data obtained from a single cell are Indicated by
black x, while a black line represents an average
FRAP curve of all analysed cells (10 cells in 2
independent experiments), also indicating the
standard deviation. To improve the readability,
error bars are shown only for every second or
fourth data point. The fluorescence intensity
measured in an unbleached region of the nucleus
is indicated by blue x, indicating the fading
during the experiment. The background
fluorescence measured outside the nucleus is
indicated by yellow x. As additional control,
FRAP data were also recorded in fixed root cells
of Arabidopsis seedlings that are indicated by
red x.
Write a Comment
User Comments (0)
About PowerShow.com