Nucleotide Structure: Pentose sugar

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Nucleotide Structure Pentose sugar
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Nucleotide Structure Nitrogenous Bases
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DNA Linkage

• Phosphate group/phosphodiester bond
• Nucleotides contain one, two, or three phosphate
  groups

nucleoside nucleotide
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Nucleic Acid Polarity

• Top of molecule has
• free 5-phosphate group
• This is the 5 end
• Bottom has a free 3
• hydroxyl group
• This is the 3 end

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Structural analysis of DNA
Early 1950s Rosalind Franklin
1953 Watson and Crick
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Complementarity
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DNA Structure
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Alternative Forms of DNA
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G C Content
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DNA Melting

• Temperature at which DNA strands are ½ denatured
  is the melting temperature or Tm
• GC content of DNA has a significant effect on Tm