What is DNA cloning how could you make a genomic library cloning 30 points

1
Quiz for Section G (25 minutes) English answer
only

• What is DNA cloning? how could you make a
  genomic library cloning? (30 points)
• What is subcloning? What are the purposes of
  subcloning. (15 points)
• Please describe all the steps when you do a
  mini-prepararation of plasmid DNA using alkaline
  lysis method. Please indicate the roles of each
  component in lysis solution and neutralization
  solution. (30 points)
• Why do you add ethidium bromide when you use CsCl
  density gradient centrifugation to purify your
  supercoiled plasmid DNA? (10 points)
• Why do you need to use alkaline phosphatase to
  treat your restricted DNA fragment when you do
  cloning and subcloning? (15 points)