IGEM TEAM EPF-LAUSANNE

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IGEM TEAM EPF-LAUSANNE
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WHAT IS IGEM?

• International Genetically Engineered Machine
• Having received a kit of biological parts from
  the iGEM Registry, we start to build our
  biological systems over the summer.
• The perspective of the Jamboree in November
  at MIT and the project design is exceptionally
  motivating, in addition to the fact that this is
  a good learning experience.

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OUR TEAM OUR SCHOOL
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THE PROJECT WET LAB PART
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THE PROJECT WET LAB PART

• Cloning Parts
• ? using the BioBrick protocol of Ginkgo
  Bioworks

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THE PROJECT MAKING A PART
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THE PROJECT WET LAB PART

• The BphP / PpsR system

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THE PROJECT MODELING PART-AIM
Goal Enhance the binary effect of the light
switch

• Molecular dynamics (MD) simulation of the
  photosensitive protein domain on computer for two
  protein states
• The light activated state
• The ground state (protein without light
  excitation)

Study of both state stability (statistical
analysis)
Determination of amino acids that could be
mutated to enhance stability of light activated
state
Mutation of the amino acids and test the switch
in vivo
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THE PROJECT MODELING PART-PRELIMINARY RESULTS

• First nanosecond MD simulation

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THE PROJECT APPLICATIONS

• Merck-Serono interests
• using light to induce gene transcription in
  mammalian cells

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• Our Mentors
• Sebastian Maerkl Laboratory of Biological
  Network Characterization (STI/SV)
• Bart Deplancke Laboratory of Systems Biology
  and Genetics
• Matteo Dal Peraro Laboratory for Biomolecular
  Modeling
• Felix Naef Computational systems biology
• THANK YOU!