Phylogeny of Euglyphid Testate Amoebae - PowerPoint PPT Presentation

About This Presentation
Title:

Phylogeny of Euglyphid Testate Amoebae

Description:

Euglypha rotunda. Euglypha rotunda. Euglypha filifera. Euglypha tuberculata. Euglypha rotunda. Euglypha rotunda. Euglypha acanthophorea. Euglypha filifera ... – PowerPoint PPT presentation

Number of Views:105
Avg rating:3.0/5.0
Slides: 7
Provided by: antonisch
Category:

less

Transcript and Presenter's Notes

Title: Phylogeny of Euglyphid Testate Amoebae


1
Phylogeny of Euglyphid Testate Amoebae
Order EUGLYPHIDA
MONADOFILOSA
2
Cloning and 18S rRNA gene sequence analysis
  • Samples 336, 337, 338 (D intact)
  • 312, 313, 314, 315 (B intermediate)
  • 1µl from each triplicate pooled gt 120 gt 1 µl for
    PCR (30c)
  • Each pooled DNA amplified 4x
  • Separate clone libraries
  • Clones screened by Amplified Ribosomal DNA
    Restriction Analysis (ARDRA)

49.1 49.9 51 52.4 54.2 56.2 57.9
59.2 61
3
Screening clones and 18S rRNA gene PCR-products
  • Amplified Ribosomal DNA Restriction Analysis
    (ARDRA)

Enzymatic digestion
  • Terminal Restriction Fragment Length Polymorphism
  • (T-RFLP)

Enzymatic digestion
100 200 300 400
4
Separating different organisms by ARDRA
MspI HinfI
5
Sequence analysis of clone libraries from 336-338
Sequence identity between clones 91-100 Sequence
identity between clones and closest related
organisms 96-97 (91)
Cluster A n 11
n 5
6
  • Ongoing work
  • Finishing comparative sequence analysis for site
    B (intermediate) and D (intact) in Chaux-dAbel
  • Testing signature sites for their suitability to
    design probes for fluorescence in situ
    hybridization (FISH) gt quantification in fixed
    samples of e.g
  • Assulina muscorum Group
  • Trinema enchelys Group
  • Euglypha acanthophora/Euglypha filifera
  • Cluster A
  • Evaluation of enzymes for T-RFLP analysis
  • Future work
  • Sequence analysis of Edwards samples gt design
    FISH probes
  • Using clones to evaluate alternative
    fingerprinting technique DGGE or SSCP
Write a Comment
User Comments (0)
About PowerShow.com