Improvement of Psidium guajava using Micropropagation

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Improvement of Psidium guajava using
Micropropagation????? ??????? ?? ???? ???????
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Aims of the Work

• Establishment of a simple and reliable in vitro
  protocol for guava micropropagation.
• Detection of Genetic Stability Using RAPD-PCR
  analysis

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Materials and Methods
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BROWNING
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Shoot formation as affected by different
combinations of growth regulators
P1 P2 P3 P4
Donor parents
IB-2
IB-4
IB-6
Regeneration media
Regeneration frequency
After 2 months
Number of shoots per explant
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Rooting induction in the regenerated guava shoots
P1 P2 P3 P4
Donor parents
I-2
I-4
N-1
N-2
Free
Rooting media
Rooting percentage
After 12 days
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The effect of successive subcultures on number of
regenerated shoots per explant
P1 P2 P3 P4
Donor parents
Subcultures
1st subculture
60 day
2nd subculture
Number of shoots per explant
30 day
3rd subculture
30 day
4th subculture
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Results
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Regeneration frequency
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A Explants on medium B After
4 weeks C After 5-6 weeks
D After 8 weeks
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Rooting percentage
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Rooting on different Auxin concentrations.
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Mass of shoots after 3 subcultures
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A Potted plants
B Field grown guava plants C flower buds
and flowers of micropropagated guava plants
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RAPD analysis
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???? ??? ????? ?? ??????? ?????? ?????? ???????
????????? 2005-2006?????? ??? ??? ???? ??? ??????
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???????? ??????? ???? ????? 2007.
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THANKS