Biocompatible%20Dextran%20coated%20Nanoceria%20with%20pH%20dependent%20Antioxidant%20properties

1
Biocompatible Dextran coated Nanoceria with pH
dependent Antioxidant properties
A. R. Asati and J. M. Perez Nanoscience
Technology Center, Department of Chemistry, and
Burnett College of Biomedical Sciences,
University of Central Florida, 12424 Research
Parkway, Suite 400, Orlando, FL 32826.
Confirmation of Dextran Coating

• Introduction
• Nanoceria is a potent free radical scavengers
  with
• neuroprotective, radioprotective,
  anti-inflammatory
• property and exhibits unique autocatalytic
  activity.
• Most of these studies have been done with
• nanoparticles with poor hydrophilicity or
  involving
• use of toxic solvents, limiting potential
  clinical
• use.
• We report a facile synthesis of monodisperse,
• water soluble and highly crystalline
  dextran-coated
• nanoceria (DNC).
• DNC exhibits unique pH-dependent antioxidant
• activity, allowing important applications in the
  design
• of improved therapeutics.

Biological studies
FT-IR
DLS Concanavallin-A
Characterization
Cell Viability Assays
Results and Conclusion Biocompatible DNC
exhibits excellent pH- dependent antioxidant
properties for improved cancer therapeutics..
Other potential applications include
development of antioxidant and anti-inflammatory
coatings in prosthetic devices and tissue
engineering and their use in supramolecular
materials. In summary, results support the
potential use of DNC as smart, pH- dependent
therapeutic agents and devices that selectively
protect healthy tissue from free radicals, which
are elevated during chronic inflammation or upon
radiotherapy or chemotherapy.
Synthesis A solution containing cerium (III)
nitrate and dextran T-10 was added drop-wise to
ammonium hydroxide solution and stirred for 24 h
at 25 ºC. The color of solution changed from
light yellow to dark brown indicating the
formation of stabilized dextran-coated ceria
nanoparticles. Afterwards, the solution
was centrifuged to settle down any debris and
large agglomerates. Finally, the centrifuged
solution was purified from free dextran and
concentrated using an amicon cell and stored at
4 ºC.
a.TEM b. HR-TEM c.XRD d. XPS e. DLS f.
Concentration
Autocatalytic Activity of DNC at pH 7.4 and pH 4.0
Acknowledgement This work was supported in part
by NCI grant CA101781 and by a UCF-NSTC Start-Up
Fund, all to Dr. J. M. Perez.
Reference J. M. Perez, A. Asati, S. Nath, C.
Kaittanis. Synthesis of biocompatible
dextran-coated nanoceria with pH-dependent
antioxidant properties. Small, Jan 2008.