NCESR 06/07-101

1
Ethanol as a Energy Source and Terminal
ReductantExploitation of Thermophilic Redox
Enzymesin Catalyst Development and Screening
Ketones
Co-PIs
David Berkowitz, Department of Chemistry
Paul Blum, School of Biological Sciences
NCESR 06/07-101
2
New Value-Added Enzymatic Reductions of Achiral
Ketones
New Biocatalytic Routes to Pharmaceutically
Relevant Building Blocks . . .
HLADH Horse Liver Alcohol Dehydrogenase CPADH
Candida parapsilosis ADH RS-1 ADH
Rhodococcus sp. ADH LKADH Lactobacillus kefir
ADH
3
Ethanol as the Terminal Reductant for Value-Added
Products
300-500 reduction cycles possible from ethanol in
the synthesis of this value-added chiral,
building block . . .
4
Thermophilic Bacteria as a Potential Source of
Novel Dehydrogenases
5
The Blum Lab successfully expresses the
thermostable ADH from the archeaon Sulfolobus
sulfataricus
6
The Berkowitz Lab finds that this thermostable
ADH can enantioselectively reduce the
Talampanel-leading ketone, even at 75C