Neuronal Differentiation of BoneMarrow Derived Mesenchymal Stem Cells in Monolayer and Seeded on bot

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Neuronal Differentiation of Bone-Marrow Derived
Mesenchymal Stem Cells in Monolayer and Seeded on
both Collagen and Chitosan Scaffolds

• Orwin Laboratory at Harvey Mudd College
• July 2006

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MSCs Differentiated into Mature Neurons in
Monolayer Cultures after 8 Days
Stains Green/Yellow - Sytox (Cell Nucleus) Red
- ?TubulinIII (Intermediate to Late Neuron
Marker) Blue - MAP2 (Mature Neuron Marker)
Purple - Colocation of ?TubulinIII and MAP 2
Key MSCStem Cells RARetinoic Acid
AstroCo-Culture w/ Astrocytes
MSC
MSC RA
MSC RA
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MSCs Co-Cultured with Astrocytes on a Collagen
Scaffold for 14 days
B
MSCs attached to collagen and expressed both
?tubulin III and MAP2. Note These are Quicktime
(.mov) Movie Files. Click to Play.
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MSCs Co-Cultured with Astrocytes on Cross-Linked
Chitosan for 14 days
MSCs attached to chitosan. MSCs express B-tubulin
and possibly MAP-2. Chitosan autofluorescence
makes it difficult to conclusively determine the
presence of MAP-2. Note These are Quicktime
(.mov) Movie Files. Click to Play.
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Cross-Section Images of Movies
Chitosan Note MAP2 signal high due to chitosan
autofluoresence and poor staining
Collagen