HHMI meeting, FOLDING

1
PROTEIN PHYSICS LECTURE 24
PROTEINS AT ACTION BIND ? TRANSFORM ? RELEASE
2
BIND repressors
?- turn - ?
3
Zn- fingers
DNA RNA BINDING
Leu-zipper
4
BIND ? TRANSFORM ? BIND Repressors
-BINDING-INDUCED DEFORMATION MAKES REPRESSOR
ACTIVE, and IT BINDS TO DNA
5
BIND Immunoglobulins
6
Immunoglobulin
7
BIND ? TRANSFORM ? RELEASE ENZYMES
chymotrypsin
Note small active site
8
Chymotrypsin catalyses hydrolysis of a peptide
Spontaneous hydrolysis very slow
9
chymotrypsin

10
CHAIN CUT-INDUCED DEFORMATION MAKES ENZYME
ACTIVE
non-active cat. site
? active cat. site
Chymotripsin Chymotripsinogen
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SER-protease catalysis
12
CHYMOTRYPSIN ACTIVE SITE with INHIBITOR
13
Catalytic antibodies ABZYM AntyBody enZYM
Transition state (TS)
Preferential binding of TS RIGID enzyme
Antibodies are selected to TS-like molecule