Breast Cancer Campaign Tissue Bank Site Visit Leeds 21409

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The Independent Cancer Patients Voice a warm
welcome to Leeds!
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Programme

• 11.15 Collection use of tissue and its value
  to cell biology
• Val Speirs, Debbie Holliday, Laura Smith, Aidan
  Hindley
• 13.00 Lunch
• with Dave Ardon, Chair NCRI Consumer Liaison
  Group
• 14.00 Bone related issues in breast cancer
  treatment
• Rob Coleman
• 15.00 Consultation on measures of emotional
  distress
• Lucy Ziegler
• 16.00 Tea ICPV discussion
• 16.30 Different methods of breast reconstruction
• Raj Achuthan
• 17.30 Lab tour
• Steven Pollock Michele Cummings

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Collection and use of tissue and its value to
cell biology

• Valerie Speirs
• Deborah Holliday
• Laura Smith

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Breast cancerOne disease or many?
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If it looks different, it is different
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310
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Frequency of chromosomal aberrations in breast
cancer
Grade I
Grade II
Roylance et al., Cancer Res 1999
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Breast cancer is heterogeneous One size does not
fit all!
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How can we model breast cancer in the lab?

• Cell lines
• Animal models
• Human clinical material

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Cell lines

• Cons
• origin
• genetic drift
• reproducibility

• Pros
• ease of use
• homogeneous
• easily replaced

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Burdall et al., Breast Cancer Res, 2003
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Case study MCF-7

• Morphology
• similar in all cases
• Growth rate
• variable
• Karyotype (CGH/SKY)
• variable
• ER/PR content
• variable

• Separate MCF-7 strains from different
  laboratories

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Animal models

• Breast cancer is a complex disease which is not
  easily modelled in animals
• Concerns over the validity of animal models
• breast tumours taken from animal experiments do
  not accurately represent human breast cancers in
  their appearance
• Ethical pressure on scientists
• 3Rs

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Primary cultures derived from human material

• Pros
• established directly from tumours
• more representative models
• Biologically
• Clinically

• Cons
• difficult to establish
• slow doubling times
• contamination by fibroblasts/normal epithelial
  cells
• Ethical issues (HTA)
• Tissue access

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Breast Cancer Campaign

• Mission
• To beat breast cancer by funding innovative
  world-class research to understand how breast
  cancer develops, leading to improved diagnosis,
  treatment, prevention and cure

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Background to Breast Cancer Campaign

• Breast Cancer Campaign is the only charity that
  specialises in funding independent breast cancer
  research throughout the UK
• Supports high quality research (basic and
  clinical) in universities, medical
  schools/teaching hospitals and research
  institutes in the UK and ROI

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• Since 1988 BCC have supported
• 183 grants
• 18.5 million
• Currently funds gt100 research projects throughout
  the UK/ROI worth gt 13.5M
• In Yorkshire, Campaign has supported
• 11 grants
• 1.25 million

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• Breast Cancer Campaign
• http//www.truveo.com/breast-cancer-campaignE280
  99s-research/id/3900128381

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Breast Cancer Campaign Gap Analysis

• One-day meeting convened in London on 2 November
  2006
• 56 of the UKs most influential breast cancer
  experts identified the key research gaps and
  priorities for the greatest potential impact on
  patients

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Format

• Before, during and after the meeting, groups in
  seven key research areas participated in cycles
  of presentation, literature review and discussion

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Groups

• Genetics
• Initiation
• Progression
• Therapies and targets
• Disease markers
• Prevention
• Psychosocial aspects

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Questions posed

• What do we know?
• What are the gaps?
• Problems
• Translational implications
• Recommendations

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• Summary papers were prepared by each group and
  collated into a position paper highlighting the
  research gaps, with recommendations for action

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Mission Statement

• To provide, in partnership with BCC, highly
  specialised breast cancer related biomaterials to
  support cutting-edge translational research for
  the benefit of the patient

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Why is the tissue bank so important?

• Access to standardised, well annotated human
  breast tissue will help identify the causes of
  breast cancer, develop new treatments, identify
  genes associated with breast cancer and, most
  importantly, accelerate research from the
  laboratory to the clinic

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Questions?