Persistence of Hunchback in the terminal region of the Drosophila blastoderm embryo impairs anterior

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Persistence of Hunchback in the terminal region
of the Drosophila blastoderm embryo impairs
anterior developmentFlorence Janody, Joachim
Reischl and Nathalie DostatniDevelopment 127,
1573-1582 (2000)
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The GAL4 UAS system

• Your first task is to describe how they
  overexpressed hunchback.
• Helpful hints
• GCN4 is a yeast transcriptional activation domain
• When fused with GAL4, it appears to help
  stabilize the expression of whatever transcript
  GAL4 activates
• Thus, the transcripts overexpression wont be
  inhibited by other factors

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How do bicoid hunchback nanos
torso function in the embryo? What would be
the phenotypes of lf mutations in these genes?
Hunchback early zygotic transcription
Torso activated at termini
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This figure shows where GAL4 (and thus hb) are
expressed in GAL4 UAS flies
persistent maternal hb
These embryos are all at a mid-cellularization
stage
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Fig. 7. Repression of hb by Torso is not
sufficient for cnc, wg and hh early anterior
expression. In situ hybridisation on blastoderm
hb -zygotic embryos with cnc, wg and hh
probes. hb -embryos were identified through their
ftz expression pattern detected with a
simultaneous staining using a ftz probe. Embryos
were from torso (A-C) and wild-type (D-F)
females.
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