AUTOLOGOUS ADULT ADIPOSE DERIVED STEM CELLS ADSC REMAINS VIABLE AFTER TRANSPLANTATION INTO RABBITS U

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AUTOLOGOUS ADULT ADIPOSE DERIVED STEM CELLS
(ADSC) REMAINS VIABLE AFTER TRANSPLANTATION INTO
RABBITS URETHRAL WALL A NEW PERSPECTIVE FOR
STRESS URINARY INCONTINENCE TREATMENT

• Almeida FG, Dantas Y, Leite K, Schor N, Ortiz V,
  Srougi M, Bruschini H.
• Federal University of São Paulo - Brazil

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STEM CELLS

• Embrionary
• Large potential
• Not autologous
• Ethical problems

• Adult (MSCs)
• Large potential
• Autologous
• Minor Ethical problems

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Adult Derived Stem Cells

• Bone Marrow
• The most studied
• Painful harvesting process
• Limited number of cells
• Search for alternatives to MSCs

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Adiposed Derived Stem Cells

• Easy to obtain
• Minimum discomfort
• Autologous transplantation
• Large number of cells
• 300 cc lipoaspirated gt 2-6 x 108 cells.
• Differentiate in several cell lineages

• Plast. Reconstr. Surg. 109 199-209, 2002
• Tissue Engineering vol. 7 (2) 212, 2001
• Plast. Reconstr. Surg. 109 199-209, 2002
• AUA annual meeting Chicago,IL 2003

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Adiposed Derived Stem Cells Urological
Applications

• UCLA- Rodriguez, LV Raz, S- (2003)
• First group to suggest ADSC as alternative on
  urological reconstructions
• ADSC injection on the rat urethra and bladder
  wall
• ADSC differentiation into Smooth Muscle cells

AUA annual meeting - Chicago,IL 2003
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OBJECTIVES

• To evaluate the feasibility of autologous
  transplantation of ADSCs into female rabbits
  urethra wall as a potential alternative for
  intrinsic urethral regeneration

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METHODSProcurement Process
Extensive washing Proteolytic digestion
Rabbits inguinal fat pad of 9 New Zealand adult
female
Minced and Washed in PBS
Centrifugation Pellet SVF
Expansion to conflluence
ADSC
Plast. Reconstr. Surg. 109 1033, 2002
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METHODSTransplantation into the urethra

• Cells labeled with Vybrant CM-DiI
• Carbocyanine
• Incorporated into cell membrane
• Injection of 30 µL of HBSS with 1 x 107 cells in
  the urethra wall of the donor rabbit
• The urethra was harvested at 2, 4 and 8 weeks
• HE stained
• Processed to identify the fluorescent DiI labeled
  cells

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Cell labeling in vitro
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4 weeks after urethral injection
200X
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2 weeks after urethral injection
100 X
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2 weeks after urethral injection
100 X
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4 weeks after urethral injection
100 X
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4 weeks after urethral injection
100 X
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4 weeks after urethral injection
100 X
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4 weeks after urethral injection
200 X
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8 weeks after urethral injection
100 X
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8 weeks after urethral injection
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8 weeks after urethral injection
100 X
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8 weeks after urethral injection
400 X
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400 X
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CONCLUSIONS

• After 8 weeks of autologous transplantation, the
  ADSCs spread and seem to incorporate into the
  urethral wall
• Due to the potential to differentiate in several
  cells lines, this technology could be useful in
  intrinsic urethral regeneration.
• The in vivo ADSCs differentiation, migration and
  function at long term need to be determinate
  before step toward clinical use.