Overview of DAR Conjugation

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Overview of DAR Conjugation
https//www.creative-biolabs.com/adc
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Introduction
Antibodies, as one main component of an
ADC(antibody-drug conjugate), plays a significant
role in target specificity and the delivery of
cytotoxic drugs. And its capability to
specifically bind to targets on the cell surface
makes sure of a successful selective delivery
system when cytotoxic drugs kill targeted cells.
Cytotoxic drugs generally refer to a payload,
which can be linked to different antibody sites
in the use of diverse conjugation in chemistry.
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DAR Conjugation
Targeted delivery of highly efficient cytotoxic
drugs is expected to improve the potential for
normal cells and tissues to reduce the risk of
target injury. Drugs that are covalently linked
are usually produced by chemical DAR conjugation.
Thus, the ADC compound should be considered a
complex mixture of different amounts of protein
molecules as well as different conjugation sites.
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Some endogenous or engineered sites for the
conjugation of cytotoxic payload drugs to an
antibody (Antibodies,2015).
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Develop enzyme-mediated modification and
conjugation system to help generate ADCs with
specific conjugation sites.
Incorporate Cysteine residues into defined sites
on an antibody to create thiol-engineered
antibody (EnCys-mAb) and conjugate payload drugs
onto those Cysteine residues via thiol-based
chemistry.
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Achieve C-terminal specific antibody modification
and yielding conjugates with DARs at 1 or 2. by
the self-splicing inteins and engineer intein-fusi
on antibodies.
Create a cave on the desired locations on an
antibody to facilitate meditope trapping through
antibody design.
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Designing a chemically compatible antibody for
the conjugation chemistry is a key step that
directly dictates the fate of an ADC project.
With expertise and dedication, advanced antibody
design platform and services from Creative
Biolabs will be your best companion in creating
customized ADCs.
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