Human Nutrition Lecture 9- Macronutrients - Fat 24 25 Homogenise with solvent (CM) Extract lipid Add a methyl ester (FAME) TAG glycerol and FFA Extract FAME into solvent like ether Report in total fatty acids based on peak areas Peaks elute at different times based on chain length and MW SCFA first Analysis by Gas Chromatography 26 (No Transcript) 27 Fatty Acids
Human Nutrition Lecture 9- Macronutrients - Fat 32 33 Trans Fatty Acids
relationship between heart disease trans - fatty acids
Ascherio Willett 1997. Health effects of trans Fatty acids AJCN 66 (suppl.) 1006S-1010S.
trans either occur naturally in dairy (from rumen) but majority produced by hydrogenation.
Human Nutrition Lecture 9- Macronutrients - Fat 33 34 Cis and Trans Fatty Acids
cis fatty acids are most natural occurring unsaturated fatty acid in diet.
cis molecules fold back in a U shape.
hydrogens are on same side of the double bond.
trans fats has hydrogens on opposite sides of the double bond.
Human Nutrition Lecture 9- Macronutrients - Fat 34 35 (No Transcript) 36 Trans Fatty Acids Food Examples
Human Nutrition Lecture 9- Macronutrients - Fat 36
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