Chemicals and Waste Disposal

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Hazard Communication
Laboratory Safety Training
Chemicals and Waste Disposal
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• This training course is divided into two parts.
• Part 1 involves a training session that covers
  basic chemical safety, a material safety data
  sheet exercise and waste management topics. Part
  1 will take approximately 30 minutes to complete.
• Once you have read through the training session
  (Part 1) you need to answer test questions (Part
  2). Part 2 will take approximately 10 minutes to
  complete. You need to get 100 correct response
  to the test questions.
• If you answer a question incorrectly, you will
  be redirected to the appropriate material to
  review. You will then need to re-answer the
  question before you can move on.

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Part 1

• Basic chemical safety, MSDS exercise and waste
  management.
• This Part will take approximately 30 minutes to
  complete.

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Federal and State Law require that all employees
who work with hazardous chemicals or potentially
infectious biological agents attend hazard
communication class. The training you are about
to do meets the requirements of the Texas Hazard
Communication Standard 502.009 and covers the
following points

• Employee Rights
• Workplace Chemical List
• Hazardous Chemicals
• Labeling
• Material Safety Data Sheets
• Emergency Response
• Waste Handling and Disposal

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Employee Rights

• You have the right to
• receive information on your exposures
• receive training on chemical hazards
• access to Material Safety Data Sheets
• (MSDS)
• receive protective equipment (free of
• charge)
• file complaints, assist inspectors, or testify
• against your employer

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Workplace Chemical List

• Is compiled and maintained by Environmental
  Health Safety for each hazardous chemical
  present in the workplace in excess of 55 gallons
  or 500 pounds.
• Includes the following information
• chemical identity used on the MSDS and container
  label
• work area where hazardous chemicals are normally
  present
• this list must be made readily available to
  employees

• Please notify EHS at 713-500-8104 if chemicals in
  excess of 55 gallons or 500 pounds are present in
  your work area.

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Many chemicals found in laboratories are
hazardous to your health. For the purpose of
worker right-to-know you will notice that
laboratory doors have signs on them indicating
the major classes of hazards associated with
working in that area.
This laboratory contains radioactive materials,
chemical agents, bio-hazardous materials and
ultraviolet light hazards.
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Due to the potential hazards, food and drink are
not permitted to be stored or consumed in
laboratories or cold/warm rooms.
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Additional Right-to-Know guidance, important
safety procedures and contact information are all
detailed in the yellow Important Safety
Information postings available in all
laboratories.
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Chemical Safety Information
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Important safety information regarding commonly
used laboratory chemicals can be found in the
following places
EHS Office Chemical Safety Binder U-T Chemical
Safety Homepage Chemical Manufacturer
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You need to know about the hazards associated
with the chemicals you are working with. The
following section will explain the important
information you will find on a chemicals label
and Material Safety Data Sheet.
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• Container Labels
• The original label from the supplier or
  manufacturer must remain attached to the
  container.
• The label provides
• name of the chemical
• manufacturers name and address
• physical and health hazard warnings

Chemical Name
Hazard Warnings
Manufacturer
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If a chemical is transferred from the original
container to a secondary container it must be
relabeled with at least the identity appearing on
the MSDS and appropriate hazard warnings.
Of course, this does not apply if the chemical is
transferred to another container for immediate
use i.e. beaker or graduated cylinder transfer.
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Material Safety Data Sheets
Material Safety Data Sheets (MSDS's) are
required for all chemicals and describe the
properties and hazards of the chemical or
product.
They are available via

• Chemical manufacturer or distributor.
• Laboratory Chemical Safety Binder these have
  been distributed to each PI.
• If unable to locate an MSDS, call EHS at
  713-500-5832 or 713-500-8100.

• MSDS link from EHS Website

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Material Safety Data Sheets

• Examples of useful sections in an MSDS
• 3. Hazards identification
• 4. First-aid measures
• 6. Accidental release measures
• 8. Exposure controls/personal protection
• 10. Stability and reactivity (storage)
• 11. Toxicological information

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Common Chemical Hazard Categories

• Flammables
• Corrosive
• Oxidizer
• Reactive

• Toxic
• Irritant
• Allergen
• Asphyxiant

Click on one of these links for detailed
information on these hazardous substances
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Physical Hazards fire, explosion Flammables
and combustibles are substances that can catch
fire and burn. Liquids (flammable) acetone,
xylene, ethanol Gas (flammable) hydrogen Solid
(flammable) metal powders, magnesium Aerosol
(flammable) spray paint -Flash point-
Flashpoint is the lowest temperature at which a
liquid has sufficient vapor pressure to form an
ignitable mixture with air near the liquids
surface. Flammable liquids have a flash point
lt100o F.
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• Health Hazards
• Acute (Immediate) Effects - headache, dizziness,
  nausea, dry and irritated skin, watery and
  stinging eyes, inflammation of eyelids.
• Chronic (Delayed) Effects - general damage to
  lungs, liver, kidneys and other systemic
  functions as well as possible cancer risk.

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Safe Handling and Storage
Keep container closed when not in use. Do not
use near open flame or ignition source
Store flammables in proper flammable storage
cabinets. acids and bases will corrode this
type of cabinet!
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Refrigerate flammables in a flammable/explosion-pr
oof refrigerator
EHS can provide a list of common-use explosion
proof refrigerators suitable for flammable liquid
storage.
Fires do occur in refrigerators from the improper
storage of and vapor accumulation from flammable
liquids!
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• A corrosive is either acidic or caustic (acid or
  base) and is capable of destroying human tissue.
  Acids can also destroy metal.
• Acids -sulfuric acid (battery acid),
• hydrochloric acid
• Bases - sodium hydroxide (drain
• cleaners), ammonium hydroxide

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Acids and bases can react violently with each
other and should be stored separately.
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Corrosives

• Health Hazards
• Acute (Immediate) Effects - Destruction of
  tissue, irritation of mucous membranes,
  respiratory irritation, difficulty breathing,
  headache, intense pain in eyes (possible loss of
  vision), nausea.
• Chronic (Delayed) Effects - Destruction of
  respiratory tract, destruction of digestive
  system.
• Special Note Acute or chronic exposure to
  hydrofluoric acid can cause deterioration of
  bones and even lead to death. Always seek
  medical attention. Go to the MSDS website for
  more information

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Oxidizers are substances that supply oxygen to
the fire as they burn.
Organic peroxides benzoyl
peroxide, perchloric acid Other
concentrated nitric acid, oxidizers
sodium hypochlorite (bleach)
Do not store oxidizers with flammables!
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REACTIVES
Reactives are compounds that are capable of
catching fire or exploding if subjected to
certain conditions.
Back Row Phosphorous Trichloride, Acetyl
Chloride, Benzoyl Chloride. Front RowThionyl
Chloride, Vinyl Chloride
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Hazards of Reactive Substances

• Fire
• Pyrophorics ignite when exposed to air (diethyl
  zinc).
• Water reactives react violently with water to
  form dangerous and flammable gases (sodium
  metal).
• Explosion
• Shock sensitive compounds can explode if shaken
  or dropped (picric acid).
• Peroxide formers form explosive compounds when
  exposed to air (ethyl ether).
• Explosives can explode if subjected to sudden
  shock, pressure, or high temperature (picric
  acid).

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TOXINS

• Acute and chronic effects of toxins vary widely.
• Read the MSDS for hazards.
• Specific hazard training can be provided through
  EHS.
• Keep Poison Control Number 1-800-POISON1
  available in
• the event of emergency.

• Storage of Toxins
• Store separately from other chemicals.
• Consider using secondary containment (plastic
  tubs).
• Restrict access to areas where toxins are stored.

Special Note Lab employees who work with
teratogens or chemicals with moderate to chronic
toxicity or high acute toxicity should refer to
the Chemical Hygiene Plan.
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Allergens
Irritants
Allergic reactions can be caused by chemicals
used in the lab or from handling organic matter
including animals. Reactions may be immediate or
delayed. Examples formaldehyde isocyanates
benzylics phenol derivatives
Irritants are materials that cause inflammation
on contact with the body surface. Irritants can
also cause changes in respiration and lung
function. Examples acetic acid sulfur
dioxidesulfuric acid formaldehyde

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Asphyxiants
Asphyxiants are chemicals that deprive body
tissues of oxygen. They are divided into two
types, simple and chemical.

• Simple asphyxiants act by diluting or displacing
  atmospheric
• oxygen, which lowers the concentration of oxygen
  in the air.
• Breathing air with low oxygen concentration can
  result in insufficient oxygen in blood and
  tissues, causing headache, unconsciousness, and
  eventually death. Inert gases, such as helium and
  nitrogen, are simple asphyxiants.
• Chemical asphyxiants prevent uptake of oxygen in
  the blood.
• Carbon monoxide, for example, binds more strongly
  to hemoglobin in the blood than oxygen does. This
  binding leaves insufficient hemoglobin available
  for oxygen uptake into the blood and transport to
  the tissues. Common chemical asphyxiants include
  hydrogen sulfide, carbon monoxide and cyanogen.

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Compressed Gas Hazards
A compressed gas is a gas or mixture of gases in
a container that is under pressure. Compressed
Nitrogen gases Compressed air Carbon
dioxide

• Ventilate area where gases are used.
• Identify cylinders by name, not color.
• Firmly close valve when not in operation.
• Keep valve protection cap on cylinder when not
  
• in use.
• Firmly secure cylinders during storage and use.
  
• For assistance, call EHS at 713-500-5832.

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• Ensure that gas cylinders are stored in
  a secured manner.
• Sudden release of gas may propel cylinder at
  high speeds with extreme force.

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MSDS Exercise

• MSDSs are required for all chemicals to help
  describe the properties and hazards of the
  chemical or product.
• The following exercise has been designed to help
  you locate important information from an MSDS.

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Material Safety Data Sheet Exercise Print out
the MSDS for acetic acid by clicking on the link
below and printing it out using your own
equipment. When you have the copy of the MSDS use
it to answer the following 6 questions
Acetic Acid MSDS
(i) Is acetic acid flammable? Yes No
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
3. Hazards Identification Emergency Overview
-------------------------- POISON! DANGER!
CORROSIVE. LIQUID AND MIST CAUSE SEVERE BURNS TO
ALL BODY TISSUE. MAY BE FATAL IF SWALLOWED.
HARMFUL IF INHALED. INHALATION MAY CAUSE LUNG AND
TOOTH DAMAGE. FLAMMABLE LIQUID AND VAPOR.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(ii) Select the chemical listed on the MSDS that
cannot be stored with acetic acid
Ethanoic acid Isopropyl alcohol Sodium
peroxide
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
10. Stability and Reactivity Incompatibilities
Acetic Acid is incompatible with chromic acid,
nitric acid, ethylene glycol, perchloric acid,
phosphorous trichloride, oxidizers, sodium
peroxide, strong caustics, most metals (except
aluminum), carbonates, hydroxides, oxides, and
phosphates.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(iii) Chronic exposure to acetic acid can lead to
what disorders?
Irritability, confusion and intolerance for
pineapples Darkening of the skin, erosion of
exposed front teeth and chronic inflammation of
the nose, throat and bronchial tubes. There
are no disorders linked to chronic exposure to
acetic acid.
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
3. Hazards Identification Potential Health
Effects ----------------------------------
Chronic Exposure Repeated or prolonged
exposures may cause darkening of the skin,
erosion of exposed front teeth, and chronic
inflammation of the nose, throat, and bronchial
tubes.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(iv) Which of the following should you do if
acetic acid is ingested?
Induce vomiting Give large quantities of water
or milk if available Administer the Heimlich
maneuver
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
4. First Aid Measures Ingestion DO NOT INDUCE
VOMITING! Give large quantities of water or milk
if available. Never give anything by mouth to an
unconscious person. Get medical attention
immediately.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(v) What is the 24-hour hotline number on the
MSDS associated with this chemical?
908-859-2151 1-800-582-3887 713-500-5832
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(vi) What kind of odor does acetic acid have?
Natural Gas Vinegar Rotten-eggs
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
9. Physical and Chemical Properties Appearance
Clear, colorless liquid. Odor Strong,
vinegar-like.
Click here to correct your answer
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Material Safety Data Sheet Exercise Continued.
(vii) Will the vapors of acetic acid go to the
floor or ceiling of a room?
Ceiling Floor
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YES! Your answer is correct
Back to the training....
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Incorrect please review this material and
correct your answer.

• 9. Physical and Chemical Properties
• Vapor Density (Air1) 2.1 (heavier than
  air-vapor would go to the floor)

Click here to correct your answer
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Fume Hood Safety
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• Keep equipment/experiments at least six inches
  inside hood.
• Keep sash at proper level when working and
  lowered when not performing work.
• If hood stops working, stop work, cover
  chemicals, close hood sash, and call
• 713-500-FIXT.
• Do not store large quantities of chemicals in
  hood.
• Airflows are verified by EHS during the routine
  laboratory safety surveys. Call 713-500-5832 for
  assistance.
• Keep hoods free of clutter.

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Personal Protective Equipment
(PPE)
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What PPE is required for you?
Always wear a lab coat when conducting work in a
laboratory. This helps to protect both you and
your clothes from chemical damage.
Latex gloves will not provide effective
protection for many chemicals. You need to know
what gloves are suitable for the hazardous agents
you are using.
Click here for more information regarding correct
glove choice.
The MSDS will tell you what type of eye
protection is required. You will be provided with
eye glasses, goggles, or mask appropriate for the
hazards associated with the agents you are using.
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Remember!
Always wear appropriate clothing and PPE while
working in the laboratory.

• A few simple rules for the Lab
• Wear closed toe shoes- no flip-flops!
• Wear long pants no shorts or skirts!
• Keep long hair tied back

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Emergency Response and Spill Clean-up
The following section will discuss what you need
to do if there is an emergency, such as a fire,
or a chemical spill in your laboratory.
Fire in a fume hood at UT 2003
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Fires can and do happen in laboratories!

• Common causes of fires include
• Improper use and storage of flammable liquids
• Unattended heat sources (Bunsen burners, hot
  plates)
• Chemical incompatibilities
• Faulty electrical wiring equipment
• Poor housekeeping-paper towels or chemicals near
  heat sources

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Types or Classes of fires
CLASS A Ordinary combustibles or fibrous
material, such as wood, paper, cloth, rubber and
some plastics.
CLASS C Energized electrical equipment, such as
laboratory appliances, switches, electrical panel
and power tools.
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Which fire extinguisher should you use?
CLASS A Fire Use ABC (dry chemical) or Class A
(pressurized water) extinguisher
CLASS B Fire Use ABC (dry chemical) or BC
(carbon dioxide) extinguisher
CLASS C Fire Use ABC (dry chemical) , Halon, or
BC (carbon dioxide) extinguisher
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Most of the fire extinguishers located throughout
UTHSC-H are the multi-use Class ABC Dry Chemical
extinguishers. You can find these in each
laboratory or in fire hose cabinets throughout
the buildings. Please click the picture to the
left to find out more important information about
this type of extinguisher.
P - Pull the Pin.A - Aim the extinguisher
nozzle at the base of the flames. S - Squeeze
trigger while holding the extinguisher upright. S
- Sweep the extinguisher from side to side,
covering the base of the fire with the
extinguishing agent.
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• Do you know the location of the safety equipment
  in and around your laboratory? Take the time to
  locate your nearest
• Fire extinguisher
• Emergency eye wash station
• Emergency showers
• Spill clean up material
• Fire alarm pull box

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Clean Up Procedures

• If a spill is an immediate threat to life or
  health
• Leave area and notify persons nearby.
• Call 911 for assistance and notify EHS at
  713-500-5832.
• Contact UTPD at 713-500-4357 (HELP)
• Provide specific and detailed information to
  responders.
• If a spill is minor
• If trained, use a spill kit to clean up. Dispose
  of waste through the hazardous waste line
  713-500-5837
• If not trained, call Chemical Safety Hotline at
  713-500-5832 for assistance.
• Prevent others from entering areas.

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All of your hazardous waste is managed by EHS in
an environmentally compliant and economic manner.
3 step process
1
Waste Generation
2
Waste pick-up
Waste processing
3
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Waste Handling and Disposal

• Waste must be stored in
• compatible containers, labeled
• and shall be kept closed except
• while filling
• Waste must be removed from
• storage within 72 hours once
• full
• Pharmaceuticals and Controlled Substances
• Call the Hazardous Waste line for guidance
• Waste pick-ups are every Monday, Wednesday and
  Friday after you call (same day pick-up if you
  call before 7 am on the pick-up day)

Waste Line 500-5837
Hazardous Waste Line713-500-5837
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Liquid Solid Waste Place waste in compatible
container, complete and attach "Hazardous Waste"
tag, call hazardous waste line
If you need hazardous waste tags or have any
questions about waste handling and disposal
please call the Waste Line 500-5837. Click here
for more information on the EPD webpage.
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No drain policy!! It is UTHSC-H policy that no
chemicals be poured down the sink.
EPD also has a chemical re-use program. Please
call 713-500-8104 for details about unused
chemicals, free of charge.
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• Broken glass
• Place in broken glass waste container if
  available or place in container or cardboard box
  labeled broken glass, seal with tape, label
  "Housekeeping" and place in hallway.

• Empty containers
• Triple rinse, place rinse in liquid waste
• Deface chemical name
• Label "Housekeeping
• Place by regular trash.

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Part 2
Test Questions. This Part will take approximately
10 minutes to complete.
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• Test
• Instructions
• There are 13 questions in this test. You must
  get 100 on the test to pass the online training.
• Read each question and answer by clicking on the
  button next to the answer you want to chose. If
  it is correct you will be taken to the next
  question, if you answer incorrectly, you will be
  asked to review the relevant material and
  re-answer the question.
• At the end of the test you will need to print
  out a copy of the EHS contact details for you
  records.
• A certificate will be sent to you at the
  conclusion of the online course via inter-office
  mail. Please keep a copy of this to verify your
  successful completion of the online training
  course.

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Click here to begin test
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Question 1 Where are the appropriate storage
locations for food and drinks (such as lunch,
snacks, soft drink and water)?
Cold rooms On a desk in a laboratory Laboratory
refrigerators Hallways, offices and designated
break areas
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
Due to the potential hazards, food and drink are
not permitted to be stored or consumed in
laboratories or coldrooms.
Click here to correct your answer
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Question 2 Where can you find safety information
for commonly used laboratory chemicals?
EHS Office Chemical Safety Binder U-T
Chemical Safety Homepage Chemical
Manufacturer All of the Above
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
Important safety information for commonly used
laboratory chemicals can be found in ALL of the
following places
EHS Office Chemical Safety Binder U-T Chemical
Safety Homepage Chemical Manufacturer
Click here to correct your answer
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Question 3 What should you do after you have
dispensed a chemical into another container that
is not intended for immediate use?
Label the new container with the identity
appearing on the MSDS and appropriate hazard
warnings to ensure that the chemical is easily
identified Write only your name or your PIs
name on the new container to ensure that no-one
uses it Nothing. Leave the container in the
laboratory and pretend you know nothing about it
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YES! Your answer is correct
Next Question
83
Incorrect please review this material and
correct your answer.
If a chemical is transferred from the original
container to a secondary container it must be
relabeled with at least the identity appearing on
the MSDS and appropriate hazard warnings.
Click here to correct your answer
84
Question 4 Which one of these categories are NOT
classified as hazardous?
Corrosive Toxic Reactive Annoying
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.

• Flammables
• Corrosive
• Oxidizer
• Reactive

• Toxic
• Irritant
• Allergen
• Asphyxiant

Click here to correct your answer
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Question 5 Which of the following are considered
when segregating and storing chemicals?
Flammability rating Reactivity Toxicity All of
the above
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
An important rule of chemical storage is to
segregate incompatible chemicals which, if
accidentally mixed, could cause fire, explosion,
or the generation of toxic gases. The MSDS for
the chemical will provide information about its
flammability rating, toxicity and reactivity to
determine what it can be stored with.
Example Acids and bases cannot be stored together
Click here to correct your answer
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Question 6 Where is a suitable storage location
for flammable liquids?
Regular laboratory refrigerator Flammable/explos
ion-proof refrigerator Next to hot plates,
Bunsen burners and other heat sources Next to
oxidizing chemicals
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
Store in flammable storage cabinets. Refrigerate
in a flammable/explosion-proof refrigerator. Do
not use near open flame or ignition source
Click here to correct your answer
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Question 7 What is an oxidizing chemical?
A chemical that supplies oxygen to breathe A
chemical that supplies oxygen to a fire as it
burns A chemical that immediately evaporates on
contact with air A chemical used to fill fire
extinguishers
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YES! Your answer is correct
Next Question
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Incorrect please review this material and
correct your answer.
Oxidizers are substances that supply oxygen to
the fire as they burn.
Click here to correct your answer
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Question 8 What safety measures must you follow
when using and /or storing compressed gas
cylinders?
Ventilate area where gases are used Identify
cylinders by name, not color Keep safety cap on
cylinder when not in use Firmly secure
cylinders during storage and use All of the
above
97
YES! Your answer is correct
Next Question
98
Incorrect please review this material and
correct your answer.

• Ventilate area where gases are used.
• Identify cylinders by name, not color.
• Firmly close valve when not in use.
• Keep valve protection cap on cylinder when not
  in use.
• Firmly secure cylinders during storage and use.

Click here to correct your answer
99
Question 9 What should you do if your fume hood
stops working?
Go home and pretend nothing happened Stop work,
cover chemicals, close hood sash, and call
713-500-FIXT. Move your work to another lab
with a working fume hood. Sounds like a good
time for a lunch break
100
Incorrect please review this material and
correct your answer.
You need to know what gloves you require for the
hazardous materials you are using. You can find
this out from the MSDS for the product.
Click here to correct your answer
101
Question 12 What is NOT appropriate lab attire?
Long pants Open-toed shoes Closed-toe
shoes Lab coat
102
YES! Your answer is correct
Next Question
103
Incorrect please review this material and
correct your answer.

• A few simple rules for the Lab
• Wear closed toe shoes- no flip-flops!
• Wear long pants no shorts or skirts!
• Keep long hair tied back

Click here to correct your answer
104
Question 13 Chemical waste should be made ready
for disposal by doing the following
Remove all identifying labels and leave uncapped
container in the hallway Take your trash to
another lab and leave to for them to take care
of Place waste in compatible container, complete
and attach "Hazardous Waste" tag, call hazardous
waste line. Throw away in regular trash when
empty
105
YES! Your answer is correct
Next Question
106
Incorrect please review this material and
correct your answer.
Liquid Solid Waste Place waste in compatible
container, complete and attach "Hazardous Waste"
tag, call hazardous waste line.
Click here to correct your answer
107
Congratulations
You will receive a certificate via inter-office
mail for successfully completing the Online Basic
Laboratory Training. Please keep a copy of this
certificate to verify your completion of this
course.
Please click on the link below to print out a
copy of emergency contact details.