MICROFLUIDICS

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MICROFLUIDICS
The new Brandeis microfluidics facility And what
it can do for you.
Wednesday, 31/08/2005   530 PM  
Volen 105   Faculty Bazaar Seth Fraden (Physics
Department, Brandeis University)
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Chemistry labs over three millenium
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wood based computation
vacuum tube computation
tokens, Susa, Iran, 3300 BC
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Microfluidic devices

• components valves, mixers,
  channels, pumps 10 100 mm in size.
• design, fabricate, and combine into a single
  instrument (lab-on-a-chip).
• precisely meter, mix, and store picoliter
  volumes of reagents.
• deliver fluids in space and time.

plumbing

• Simple to fabricate Microfluidic devices
  composed of an elastomeric polymer (PDMS).

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Whitesides, Soft lithography
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Flow Focusing
Phase Chip

• 50 pl drops
• 30 micron nozzle
• 60 micron channel

oil
aqueous
High speed camera 5000 frames/sec
oil

• 50x time lapse 40 drops / sec

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b
200 mm
co
-
existence
co
-
existence
b
b
precipitant concentration
precipitant concentration
c

stable
stable
c
c
solution
solution
b

a
a
crystal
c

protein concentration
nucleate and grow crystal
dissolve crystal
15 mm dialysis membrane
exchange channels
PDMS
Dissolve Crystal Low salt solution in exchange
channels
Crystallization Air in exchange channels
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MICROFLUIDICS
The new Brandeis microfluidics facility And what
it can do for you.
Wednesday, 31/08/2005   530 PM  
Volen 105   Faculty Bazaar Seth Fraden (Physics
Department, Brandeis University)